Effects of high temperature on insecticide tolerance in whitefly Bemisia tabaci (Gennadius) Q biotype

Bemisia tabaci (Gennadius) Q biotype (BTQ) has spread to many tropical and subtropical regions over the past several decades. This may reflect an advantage biotype Q has over closely related forms in having greater thermal and/or insecticide resistance, although the effects of higher temperatures on insecticide tolerance of BTQ has, to date, been largely ignored. In this study, the effects of elevated temperatures on BTQ's tolerance to the insecticide thiamethoxam were investigated. The effect on the activities of detoxifying enzymes [carboxylesterase (CarE), glutathione S-transferase (GST), and cytochrome P450 monooxygenase (P450)] and expression profiling of eleven genes of detoxifying enzymes were also determined. In addition, RNA interference (RNAi) and bioassay methods were used to further identify the function of CYP6CM1 in tolerance to thiamethoxam following exposure to higher temperatures. The results showed that elevated temperatures were responsible for causing different outcomes in the tolerance of BTQ to thiamethoxam: Temperatures of 35 °C or higher decreased the tolerance of BTQ to thiamethoxam, while a moderately high temperature of 31 °C increased the tolerance. The high temperature influenced the tolerance of BTQ by affecting the activity of P450. Quantitative real-time PCR (qPCR) showed that CYP6CM1 was significantly up-regulated in most treatments at 31 °C, but was suppressed at 35 °C, which was closely associated with the mortality rates. Feeding on double-stranded RNA (dsRNA) of CYP6CM1 significantly reduced the mRNA levels of the target gene in the adults, and dramatically decreased tolerance to thiamethoxam induced by a temperature of 31 °C for 6 h. Our finding provides useful information to better understand the invasion mechanism of BTQ.