Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rat ventral prostate cell lysis at 2 degrees C.

K M Anderson, M Rubenstein

    Clinical Physiology and Biochemistry
    |January 1, 1986
    PubMed
    Summary

    Mechanical dissociation of rat prostate tissue at 2°C causes cell lysis, impacting epithelial and connective tissue cells. This study investigates the properties of this potent cell-associated lytic event.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Implementation strategies to support pre-exposure prophylaxis for HIV prevention care for cisgender women in federally-funded family planning clinics in Atlanta, Georgia.

    Frontiers in reproductive health·2025
    Same author

    Publisher Correction: Brain charts for the human lifespan.

    Nature·2022
    Same author

    Brain charts for the human lifespan.

    Nature·2022
    Same author

    Comment.

    Statistics in biopharmaceutical research·2018
    Same author

    Clustering of clinical and environmental Escherichia coli O104 isolates using the DiversiLab™ repetitive sequence-based PCR system.

    Current microbiology·2014
    Same author

    Conducting systematic reviews of intervention questions I: Writing the review protocol, formulating the question and searching the literature.

    Zoonoses and public health·2014

    Area of Science:

    • Cell biology
    • Prostate research

    Background:

    • Separating epithelial from nonepithelial cells is crucial for prostate research.
    • Previous methods for cell isolation yielded low intact cell counts.

    Purpose of the Study:

    • To investigate the mechanical dissociation of rat ventral prostate.
    • To characterize a cell-associated lytic event affecting prostate cells during dissociation.

    Main Methods:

    • Rat ventral prostate tissue minced at 2°C.
    • Tissue dissociation via sieving and washing.
    • Cell separation using Ficoll gradient centrifugation.

    Main Results:

    • Limited separation of intact prostate cells was achieved.
    • Mechanical dissociation at 2°C induced rapid lysis of epithelial and connective tissue cells.
    • Cell nuclei and blood cells remained largely intact, suggesting a cell-specific lytic mechanism.

    Conclusions:

    • Mechanical dissociation of rat ventral prostate at low temperatures triggers a potent cell-associated lytic event.
    • This lysis affects prostate cells but not their nuclei or circulating blood cells.
    • Further characterization of this lytic process is warranted.

    Related Experiment Videos