Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:26

Membrane Fluidity

15.6K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
15.6K
Membrane Fluidity01:23

Membrane Fluidity

174.5K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
174.5K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

7.2K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
7.2K
Adhesion01:14

Adhesion

44.5K
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
44.5K
Kinetic Energy00:23

Kinetic Energy

43.5K
Kinetic energy is the ability of an object in motion to do work or enact change. It can take on many forms. For instance, water flowing down a waterfall has kinetic energy. In biological systems, particles of light travel and are absorbed by plants to create chemical energy. Animals consume the chemical energy and give off molecules that carry their scent through the air. They also generate kinetic energy when they run away from predators. Entire systems also possess kinetic energy, like the...
43.5K
Enzyme Kinetics01:19

Enzyme Kinetics

104.2K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
104.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Record-high Glass coefficient in the shift current response of a ferroelectric halide perovskite.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Oxygenated Perfusion Enhances Hepatocyte Function in Human iPSC-liver Tissue.

Transplantation·2026
Same author

Evaluation of the Spatial Distribution of Temperature of Aqueous Solutions under the Conditions of Laser Trapping for Molecular Assembly.

The journal of physical chemistry. B·2026
Same author

Spatiotemporal Control of Formation of Dynamic Protein Fiber Assemblies via Photophysical Effects of a Focused Laser Beam.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

COVID-19-related anxiety and sense of coherence associated with psychological distress among rural residents in Japan during the pandemic late phase.

Environmental health and preventive medicine·2026
Same author

Author Correction: Ultra-low core loss in Fe-enriched soft magnetic ribbons enabled by nanostructure and high-frequency domain engineering.

Nature communications·2026

Related Experiment Video

Updated: Feb 5, 2026

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

1.6K

Defining Lineage-Specific Membrane Fluidity Signatures that Regulate Adhesion Kinetics.

Takahisa Matsuzaki1, Shinya Matsumoto2, Toshiharu Kasai2

  • 1Institute of Research, Tokyo Medical and Dental University (TMDU), 15-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan; Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Kanazawa-ku 3-9, Yokohama, Kanagawa 236-0004, Japan.

Stem Cell Reports
|September 11, 2018
PubMed
Summary
This summary is machine-generated.

Cellular membrane fluidity changes during stem cell differentiation. Researchers developed a novel method, Adhesion Sorting (AdSort), to purify specific cell types by manipulating this fluidity.

Keywords:
cell adhesioncell sortingfluidic modulatormembrane fluiditypluripotency

More Related Videos

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
13:22

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Published on: November 2, 2011

15.4K
Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
13:26

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

Published on: March 31, 2018

10.3K

Related Experiment Videos

Last Updated: Feb 5, 2026

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

1.6K
Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
13:22

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Published on: November 2, 2011

15.4K
Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
13:26

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

Published on: March 31, 2018

10.3K

Area of Science:

  • Stem Cell Biology
  • Cellular Biophysics
  • Developmental Biology

Background:

  • Cellular membrane fluidity is crucial for cell adhesion and migration.
  • Understanding lineage-specific membrane fluidity during differentiation is essential.

Purpose of the Study:

  • To systematically map membrane fluidity landscapes across human stem cell differentiation.
  • To investigate the role of membrane composition in signaling and lineage specification.
  • To develop a novel cell purification strategy based on membrane fluidity modulation.

Main Methods:

  • Characterization of membrane fluidity across various human pluripotent and differentiated cell lineages.
  • Analysis of membrane composition impact on activin signaling.
  • Development and screening of over 1,150 conditions for the label-free Adhesion Sorting (AdSort) method.

Main Results:

  • Membrane rigidification precedes the exit from pluripotency.
  • Membrane composition influences activin signaling transmission.
  • Germ layer-specific membrane fluidity signatures were identified, linked to lipid compositions.
  • The AdSort method effectively eliminates pluripotent stem cells and purifies target populations.

Conclusions:

  • Tunable membrane fluidity plays a significant role in stem cell maintenance and differentiation.
  • Modulating membrane fluidity offers a viable strategy for lineage-specific cell purification.