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Development of New Therapeutic Applications Using Microfluidics
08:56

Development of New Therapeutic Applications Using Microfluidics

Published on: October 1, 2007

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Application of

Azar Dokht Khosravi1,2, Mitra Roointan2, Effat Abbasi Montazeri2

  • 1Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Infection and Drug Resistance
|September 11, 2018
PubMed
Abstract

Insights

Coagulase-negative staphylococci (CoNS) are significant nosocomial pathogens. This study identified prevalent CoNS species and high rates of methicillin-resistant CoNS (MRCoNS), highlighting the need for control measures.

Area of Science:

  • Microbiology
  • Clinical Infectious Diseases
  • Genetics

Background:

  • Coagulase-negative staphylococci (CoNS) are common skin inhabitants but also major nosocomial pathogens, particularly in immunocompromised individuals.
  • Increasing incidence of CoNS, especially methicillin-resistant strains (MRCoNS), necessitates accurate species-level identification.
  • The tuf gene is a reliable marker for CoNS species identification.

Purpose of the Study:

  • To identify CoNS species using tuf gene-based PCR and sequencing.
  • To determine the prevalence of MRCoNS and other antibiotic resistances in clinical isolates.

Main Methods:

  • Collected 200 staphylococci isolates from clinical samples.
  • Used phenotyping for initial identification, followed by tuf gene PCR and sequencing.
  • Analyzed phylogenetic relationships using the neighbor-joining method.
  • Assessed methicillin resistance via microbroth dilution and antibiotic susceptibility using disk diffusion.

Main Results:

  • Identified 125 CoNS isolates, with Staphylococcus epidermidis (43.2%) and Staphylococcus haemolyticus (40.0%) being most common.
  • Detected methicillin resistance in 54.4% of CoNS isolates.
  • High resistance observed for cefoxitin (65.4%), cotrimoxazole (54.4%), and clindamycin (49.6%); daptomycin and linezolid showed high effectiveness.

Conclusions:

  • Confirmed S. epidermidis and S. haemolyticus as predominant CoNS species.
  • The high prevalence of MRCoNS is a significant concern, demanding hospital control measures.
  • Urgent need to reduce MRCoNS infections, especially in immunocompromised patients.
Keywords:
PCRantimicrobial resistancecoagulase-negative staphylococcisusceptibility testingtuf gene

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