Enantiomers separation by capillary electrochromatography using polysaccharide-based stationary phases

Area of Science:

• Analytical Chemistry
• Pharmaceutical Science
• Biochemistry

Background:

• Chiral compounds are vital in biological processes and human health.
• Enantiomers in drugs can exhibit varying pharmacological activity or toxicity.
• Accurate separation of enantiomers is critical for drug analysis and safety.

Purpose of the Study:

• To review capillary electrochromatography (CEC) as a technique for chiral separation.
• To highlight the role of polysaccharide derivatives as chiral stationary phases (CSPs).
• To present practical applications of CEC with polysaccharide CSPs in enantiomer resolution.

Main Methods:

• Review of scientific literature on capillary electrochromatography.
• Focus on the principles of CEC, including electroosmotic flow and stationary phase interactions.
• Analysis of polysaccharide derivatives utilized as CSPs for chiral recognition.

Main Results:

• Capillary electrochromatography offers high separation efficiency and selectivity for chiral compounds.
• Polysaccharide-based chiral stationary phases are effective for resolving enantiomers.
• CEC with polysaccharide CSPs has demonstrated practical utility in various drug analysis scenarios.

Conclusions:

• Capillary electrochromatography is a powerful technique for chiral separation.
• Polysaccharide derivatives serve as versatile and effective chiral stationary phases.
• This approach is highly valuable for enantiomeric analysis in pharmaceutical research and quality control.