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The Effect of Permethrin Resistance on

Liming Zhao1, Barry W Alto2, Dongyoung Shin3

  • 1Florida Medical Entomology Laboratory, University of Florida, 200 9th Street South East, Vero Beach, FL 32962, USA. lmzhao@ufl.edu.

Viruses
|September 12, 2018
PubMed

Insights

Insecticide resistance in Aedes aegypti mosquitoes, the primary arbovirus vector, was studied using RNA-sequencing. Zika virus infection altered gene expression in resistant mosquitoes, highlighting potential P450 targets for control.

Area of Science:

  • * Molecular biology and entomology, focusing on vector-borne diseases and insecticide resistance.

Background:

  • * Aedes aegypti mosquitoes are significant vectors for emerging arboviruses like Zika.
  • * Insecticide resistance in mosquito populations complicates disease control efforts.

Purpose of the Study:

  • * To compare transcriptomes of permethrin-resistant and susceptible Aedes aegypti strains after Zika virus infection.
  • * To identify molecular mechanisms underlying insecticide resistance and Zika virus interactions.

Main Methods:

  • * RNA-sequencing to analyze gene expression differences between resistant and susceptible mosquito strains.
  • * Quantitative real-time PCR to validate gene expression changes in response to Zika virus.

Main Results:

  • * 2459 transcripts showed significant expression differences between resistant and susceptible Aedes aegypti.
  • * Highly overexpressed P450 enzymes (AAEL014617, AAEL006798) were identified as potential contributors to permethrin resistance.
  • * Zika virus infection altered gene expression of detoxification and immune enzymes in resistant mosquitoes.

Conclusions:

  • * Zika virus infection impacts gene expression in insecticide-resistant Aedes aegypti.
  • * Understanding these molecular interactions can inform novel mosquito and arbovirus control strategies.
Keywords:
Aedes aegyptiRNA-seqZika virusdetoxification and immune system responsesinsecticide resistance

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