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Camila Quinello1, Lucas Souza Ferreira2, Isabella Picolli3

  • 1Department of Clinical Analysis, School of Pharmaceutical Sciences, Araraquara, São Paulo State University (UNESP), Rodovia Araraquara-Jaú-Km 1, Araraquara 14800-903, SP, Brazil. caquinello@hotmail.com.

Journal of Fungi (Basel, Switzerland)
|September 12, 2018
PubMed
Summary

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This study explores using dendritic cells (DCs) stimulated with Sporothrix schenckii cell wall proteins to combat sporotrichosis. These activated DCs promote a protective immune response, suggesting potential for new prophylactic treatments.

Area of Science:

  • Immunology
  • Mycology
  • Vaccinology

Background:

  • Sporotrichosis is a fungal infection with limited treatment options, especially for immunocompromised individuals.
  • Dendritic cells (DCs) are key antigen-presenting cells with potential in vaccination strategies.
  • Alternative therapies are needed due to the inefficiency of conventional antifungal treatments.

Purpose of the Study:

  • To evaluate the potential of bone-marrow-derived dendritic cells (BMDCs) stimulated with Sporothrix schenckii cell wall proteins (SsCWP) as a prophylactic tool against sporotrichosis.
  • To analyze the immune profile induced by SsCWP-stimulated BMDCs.

Main Methods:

  • Mouse BMDCs were stimulated with SsCWP.
  • Surface expression of costimulatory molecules (CD80, CD86, CD40) and TLR-4 was analyzed.
Keywords:
Sporothrix schenckiibone-marrow-derived dendritic cellssporotrichosisvaccine

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  • Cytokine secretion (IL-6, IL-17A, TNF, IL-10, IFN-γ, IL-2) was measured in BMDCs and in cocultures of BMDCs with splenocytes.

    • Main Results:

    • SsCWP-stimulated BMDCs showed increased expression of CD80, CD86, and CD40, but not TLR-4.
    • Activated BMDCs secreted higher levels of IL-6, IL-17A, and TNF.
    • Coculture with splenocytes resulted in elevated IFN-γ, IL-10, and IL-2, with altered secretion of other cytokines, suggesting a Th1-prone immune profile.

    Conclusions:

    • SsCWP-stimulated BMDCs induce a Th1-prone cytokine profile, which is associated with protection against fungal diseases.
    • This finding supports the development of DC-based prophylactic or therapeutic strategies for sporotrichosis.