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Muhammad Sohail Anjum1, Azra Mehmood, Faiza Mahmood

  • 1National Centre of Excellence in Molecular Biology, University of Punjab, 87-West Canal Bank Road, Lahore, Pakistan.

Journal of Biosciences
|September 13, 2018
PubMed
Summary
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Pretreating stem cells with growth factors protects them from high glucose. This enhanced insulin-producing cell therapy may improve diabetes treatment outcomes.

Area of Science:

  • Stem cell biology
  • Diabetes research
  • Regenerative medicine

Background:

  • Diabetic pancreas oxidative stress impairs transplanted stem cell viability.
  • Growth factor pretreatment can enhance stem cell stress tolerance.
  • Adipose-derived mesenchymal stem cells (ADMSCs) can differentiate into insulin-producing cells (IPCs).

Purpose of the Study:

  • To investigate the protective effect of combined stromal cell-derived factor 1 alpha (SDF1α) and basic fibroblast growth factor (bFGF) pretreatment on ADMSC-derived IPCs against hyperglycaemic stress.

Main Methods:

  • ADMSCs were differentiated into IPCs.
  • IPCs were pretreated with a combination of SDF1α and bFGF.
  • Pretreated and control IPCs were exposed to hyperglycaemic conditions (17 mM or 33 mM glucose).

Related Experiment Videos

  • Apoptosis, senescence, cell damage, insulin release, cell proliferation, and gene expression (Insulin 1, Insulin 2, Ngn3, Pdx1, Nkx6.2) were assessed.

    • Main Results:

    • SDF1α and bFGF pretreated IPCs showed significantly reduced apoptosis, senescence, and cell damage under 33 mM glucose stress.
    • Pretreated IPCs exhibited increased insulin release and enhanced cell proliferation compared to controls.
    • Upregulation of key beta-cell related genes (Insulin 1, Insulin 2, Ngn3, Pdx1, Nkx6.2) was observed in pretreated IPCs.

    Conclusions:

    • Combined SDF1α and bFGF pretreatment confers significant protection to ADMSC-derived IPCs against hyperglycaemic stress.
    • This enhanced stem cell therapy holds promise for improving therapeutic outcomes in diabetes treatment.