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Comparative Lesions Analysis Through a Targeted Sequencing Approach
Published on: November 5, 2019
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Reference method for digital surface measurement of target lesions in vitiligo: a comparative analysis
N van Geel1, D Vandendriessche1, E Vandersichel1
1Department of Dermatology, Ghent University Hospital, Ghent, Belgium.
The British Journal of Dermatology
|September 13, 2018
Summary
ImageJ functions provide reliable and valid measurements for vitiligo target lesions. Transparent sheets with the ImageJ wand function are recommended as a reference method for future clinical trials.
Area of Science:
- Dermatology
- Medical Imaging
- Biomedical Engineering
Background:
- Objective measurement of vitiligo lesions is crucial for clinical practice and trials.
- Existing digital tools have limitations in feasibility, validity, reliability, and responsiveness.
- Current studies often do not utilize ultraviolet (UV) photography for vitiligo assessment.
Purpose of the Study:
- To evaluate the reliability, validity, and feasibility of two ImageJ functions for measuring vitiligo target lesions.
- To assess these functions using various imaging types, including UV photography.
- To establish a potential reference method for future vitiligo measurement studies.
Main Methods:
- Planimetric measurements were conducted on 52 vitiligo lesions from 10 patients.
- Measurements were performed on photographs (with and without UV) and transparent sheets.
- ImageJ 'wand' and 'threshold' functions were utilized by multiple assessors to evaluate reliability and validity.
Main Results:
- Both ImageJ 'wand' and 'threshold' functions demonstrated excellent inter- and intrarater reliability (ICC > 0.9) on photographic images.
- Measurements on transparent sheets yielded the highest agreement (ICC > 0.95) and lowest variance.
- All construct validity hypotheses were confirmed, and user-friendliness was satisfactory, with transparent sheets being faster to measure.
Conclusions:
- The study validates the reliability, validity, and feasibility of ImageJ functions for measuring vitiligo target lesions.
- Transparent sheets measured with the ImageJ 'wand' function are proposed as a reference method for future trials.
- This method can aid in validating other digital instruments for vitiligo assessment.
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