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Updated: Feb 5, 2026

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
Published on: May 2, 2018
Development of a new method for biomonitoring of multiple metals in occupational exposure
Seyed Mohammad Sadat1, Shirin Seyed Ghoreyshi1, Fatemeh Rajabi1
1a Environmental and Occupational Hazards Control Research Center , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
Abstract:
The assessment of co-exposure to several types of metal contamination poses a hurdle for occupational monitoring. Determination of elements in biological samples is an important way to evaluate occupational exposure. However, optimized methods for the extraction of multiple metals from biological samples have not been reported in recent studies. Therefore, solid-phase extraction (SPE) based on the functionalized nano-zeolite Y was suggested for the biomonitoring of metal co-exposure. SPE was conducted with ammonium pyrrolidine dithiocarbamate (APDC) surrounded by Triton X-100 micelles, which were loaded into the pores of nano-zeolite Y. In this study, SPE was optimized for pre-concentration of trace amounts of chromium (Cr), cadmium (Cd), and lead (Pb) in urine samples with respect to the pH, APDC concentration, elution condition, amount of functionalized nano-zeolite Y, and sample volume. This method has been successfully optimized for the extraction of the mentioned multiple metals with >97% efficiency and an acceptable reproducibility with a coefficient variation of <10%. This method could be used in the extraction of multiple metals in environmental and occupational exposure conditions. In this study, urine samples of welding workers were evaluated following this optimized method.
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