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Related Experiment Videos

Leyun Yang1,2, Cheng Zheng1,2, Yong Chen1,2

  • 1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.

Frontiers in Microbiology
|September 14, 2018
PubMed
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The transcription factor MIG1 is crucial for Saccharomyces cerevisiae biofilm formation and ethanol resistance during industrial fermentation. Deleting MIG1 weakens biofilm formation and reduces ethanol tolerance, impacting fuel production efficiency.

Area of Science:

  • Industrial biotechnology
  • Microbial physiology
  • Bioprocess engineering

Background:

  • Saccharomyces cerevisiae immobilization enhances ethanol fuel production.
  • Biofilm formation increases yeast ethanol stress resistance.
  • Mechanisms of yeast biofilm formation and stress resistance are not fully understood.

Purpose of the Study:

  • Investigate the role of the transcription factor MIG1 in yeast biofilm formation.
  • Determine MIG1's contribution to ethanol resistance in Saccharomyces cerevisiae biofilms.
  • Elucidate MIG1's regulatory role in adhesion gene expression during fermentation.

Main Methods:

  • Transcriptome analysis of biofilm and planktonic Saccharomyces cerevisiae cells.
  • Construction and analysis of MIG1 deletion mutant strains.
Keywords:
Saccharomyces cerevisiaebiofilmethanol resistanceflocculation genestranscription factor MIG1

Related Experiment Videos

  • Quantitative reverse transcription PCR (qRT-PCR) to measure FLO gene expression.
  • Assessment of biofilm formation, flocculation, invasive growth, and ethanol tolerance.

    • Main Results:

    • MIG1 expression increases during yeast biofilm formation.
    • MIG1 deletion mutants exhibit reduced biofilm formation and ethanol tolerance.
    • MIG1 regulates the expression of adhesion genes FLO1, FLO5, and FLO9, impacting cell-cell and cell-substrate adhesion.

    Conclusions:

    • MIG1 is essential for robust yeast biofilm development and enhanced ethanol tolerance.
    • MIG1-mediated regulation of FLO genes contributes to cell adhesion and environmental resilience.
    • Understanding MIG1's role can optimize immobilized yeast fermentation for industrial ethanol production.