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The construction of a root locus involves several key steps to analyze and visualize the behavior of a system's poles with varying gain. The number of branches in the root locus equals the number of closed-loop poles and is symmetrical about the real axis.
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Related Experiment Video

Updated: Feb 5, 2026

Construction and Characterization of a Novel Vocal Fold Bioreactor
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Construction, expression, and characterization of AG1

Xie Yan1, Yan-Tao Yang1, Wei Shi1

  • 1College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

Data in Brief
|September 14, 2018
PubMed
Summary

Researchers developed a new method to construct and express difficult DUF1220 family proteins, specifically AG11-843 and AG11-1581, overcoming challenges with their complex DNA sequences for broader scientific application.

Keywords:
AG1DUF1220Gene synthesisPCRPlasmid constructionRecombinant protein expression

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Area of Science:

  • Molecular Biology
  • Protein Expression
  • Biochemistry

Background:

  • The DUF1220 protein family plays a significant role in biological processes.
  • Recombinant protein production for DUF1220 family members is challenging due to complex DNA sequences.
  • AG1 is a key member of the DUF1220 protein family.

Purpose of the Study:

  • To develop an effective technique for constructing and expressing proteins with complicated DNA sequences.
  • To successfully produce and characterize recombinant AG1 variants (AG11-843 and AG11-1581).

Main Methods:

  • DNA sequence optimization and design.
  • Cloning using overlap PCR.
  • Construction into an expression vector.
  • Overexpression in Escherichia coli.
  • Protein purification.
  • Characterization via dynamic light scattering and gel filtration analysis.

Main Results:

  • Successfully optimized the DNA sequence for AG1 variants.
  • Constructed and expressed AG11-843 and AG11-1581 in E. coli.
  • Purified the recombinant proteins.
  • Characterized the proteins using biophysical methods.

Conclusions:

  • An effective method was established for constructing and expressing proteins with complex DNA sequences.
  • This technique facilitates the study of challenging proteins like DUF1220 family members.
  • The generated data provides a foundation for further research on AG1 and related proteins.