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A Microfluidic Chip for ICPMS Sample Introduction
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Microfluidic Based Optical Microscopes on Chip.

Petra Paiè1, Rebeca Martínez Vázquez1, Roberto Osellame1,2

  • 1Istituto di Fotonica e Nanotecnologie, Consiglio Nazionale dell Ricerche, Piazza Leonardo da Vinci 32, 20133 Milan, Italy.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|September 14, 2018
PubMed
Summary
This summary is machine-generated.

Optofluidics enables lab-on-chip devices for cell analysis. This review explores on-chip microscopy techniques, including bright-field, phase contrast, holographic, and fluorescence microscopy, for enhanced biological sample monitoring.

Keywords:
brightfieldfluorescencelab on chipoptical microscopyoptofluidics

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Area of Science:

  • Optofluidics and Microfluidics
  • Biomedical Imaging
  • Lab-on-Chip Technology

Background:

  • Advancements in optofluidics have led to integrated lab-on-chip (LOC) devices for cell culture, analysis, and manipulation.
  • Optical imaging is crucial for monitoring biological samples in microfluidic systems but traditionally requires bulky bench-top microscopes.
  • Implementing optical imaging directly within LOC systems enhances automation, compactness, and portability.

Purpose of the Study:

  • To review existing solutions for integrating microscopy capabilities into lab-on-chip devices.
  • To discuss various optical imaging techniques applicable for on-chip biological sample analysis.
  • To highlight the benefits of on-chip microscopy for microfluidic applications.

Main Methods:

  • Discussion of integrated optical elements within microfluidic devices.
  • Exploration of lensless imaging methods for on-chip applications.
  • Review of different microscopy techniques adapted for LOC systems, including bright-field, phase contrast, holographic, and fluorescence microscopy.

Main Results:

  • Successful implementation of various optical microscopy techniques directly onto microfluidic chips.
  • Demonstration of enhanced automation, compactness, and portability in LOC imaging systems.
  • Availability of diverse imaging modalities (bright-field, phase contrast, holographic, fluorescence) for on-chip analysis.

Conclusions:

  • On-chip microscopy represents a significant advancement for microfluidic biological sample analysis.
  • Integrated optical imaging solutions offer improved efficiency and accessibility for cell analysis and manipulation.
  • The reviewed techniques pave the way for more sophisticated and portable microfluidic diagnostic and research tools.