Interaction of colony stimulating factor (CSF) from different sources with sepharose-concanavalin A

Preparations from various sources (human urines, mouse placenta and pregnant uterus extract, and fibroblast conditioned medium) were examined comparatively with affinity chromatography on Sepharose-Concanavalin A for the presence of Colony Stimulating Factor (CSF). The preparations from all sources constantly revealed two protein peaks: the proteins of the first peak, without Concanavalin A affinity, contained CSF in the material from pregnant uterus and fibroblast conditioned medium, while human urine preparation was devoid of CSF: the inhibitor of CFUc growth, detected and titrated in this peak, probably does not explain the lack of CFUc growth. The proteins from the second peak, specifically eluted with alpha-methyl D-glucopyranoside revealed CSF in all the examined preparations.