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Quantitative PCR of T7 Bacteriophage from Biopanning

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Published on: September 27, 2018

Simple drop cast method for enumeration of bacteriophages.

Sanjay Chhibber¹, Prabhjot Kaur¹, Vijay Singh Gondil¹

¹Department of Microbiology, Basic Medical Sciences, Panjab University, Chandigarh, India.

Journal of Virological Methods

|September 15, 2018

Summary

A new drop cast method offers an easier, cost-effective way to enumerate bacteriophages (phages). This technique allows multiple dilutions on one plate, providing a reliable first approximation of phage counts.

Keywords:

Bacteriophages Phage therapy Plaque assay Soft agar

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Area of Science:

Microbiology
Biotechnology

Background:

Phage enumeration is crucial for industrial and medical applications.
The classical Double Layer Agar (DLA) plaque assay is standard but cumbersome.

Purpose of the Study:

To introduce a simpler, more cost-effective method for phage enumeration.
To compare the proposed drop cast method with the traditional DLA assay.

Main Methods:

A novel drop cast method for phage enumeration was developed.
This method uses a single agar layer without strict temperature controls.
Multiple phage dilutions are plated on a single agar plate.

Main Results:

The drop cast method is easier and more cost-effective than DLA.
It allows enumeration of 7-10 dilutions per plate, unlike DLA's one dilution.
While standard deviation was higher than DLA, it provides a reliable first approximation.

Conclusions:

The drop cast method is a reliable, easy, and cost-effective technique for approximate phage enumeration.
It serves as a valuable preliminary method, with results potentially validated by DLA for higher accuracy.