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Updated: Feb 5, 2026

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format
Published on: December 5, 2025
Link between carrot leaf secondary metabolites and resistance to Alternaria dauci
Claude Koutouan1, Valérie Le Clerc2, Raymonde Baltenweck3
1IRHS, Université d'Angers, Agrocampus-Ouest, INRA, SFR 4207 QuaSaV, 49071, Beaucouzé, France.
Abstract:
Alternaria Leaf Blight (ALB), caused by the fungus Alternaria dauci, is the most damaging foliar disease affecting carrots (Daucus carota). In order to identify compounds potentially linked to the resistance to A. dauci, we have used a combination of targeted and non-targeted metabolomics to compare the leaf metabolome of four carrot genotypes with different resistance levels. Targeted analyses were focused on terpene volatiles, while total leaf methanolic extracts were subjected to non-targeted analyses using liquid chromatography couple to high-resolution mass spectrometry. Differences in the accumulation of major metabolites were highlighted among genotypes and some of these metabolites were identified as potentially involved in resistance or susceptibility. A bulk segregant analysis on F3 progenies obtained from a cross between one of the resistant genotypes and a susceptible one, confirmed or refuted the hypothesis that the metabolites differentially accumulated by these two parents could be linked to resistance.
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