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Related Experiment Videos

Both normal and tumor cells produce basic fibroblast growth factor.

D Moscatelli, M Presta, J Joseph-Silverstein

    Journal of Cellular Physiology
    |November 1, 1986
    PubMed
    Summary

    Basic fibroblast growth factor (FGF) stimulates cell growth and blood vessel formation. This study found FGF-like molecules in various cell lines, suggesting a widespread role in cell function.

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    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • Basic fibroblast growth factor (FGF) is a molecule known to stimulate various cellular processes.
    • Its role in endothelial cell function, including plasminogen activator (PA) production and angiogenesis, is of significant interest.

    Purpose of the Study:

    • To identify and characterize basic FGF-like molecules in cultured normal and tumor-derived cells.
    • To investigate the potential autocrine role of basic FGF in bovine capillary endothelial (BCE) cells.

    Main Methods:

    • Purification of basic FGF-like molecules from human placenta.
    • Assay for PA production in BCE cells to detect FGF-like activity.
    • Characterization of activity using heparin affinity and antibody cross-reactivity.

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  • Screening of ten cell lines for FGF-like molecules.

    • Main Results:

    • Basic FGF-like molecules were identified in eight out of ten tested cell lines.
    • The presence of FGF-like activity did not correlate with the cell's origin (normal vs. tumor).
    • BCE cells exhibited high levels of FGF-like activity, indicating a potential autocrine function.

    Conclusions:

    • Basic FGF-like molecules are widely distributed in various cultured cell lines.
    • These molecules possess biological activities including stimulation of PA production, DNA synthesis, motility, and angiogenesis.
    • BCE cells may utilize basic FGF in an autocrine manner for self-regulation.