Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Reduced Coenzymes NADH and FADH₂01:29

Role of Reduced Coenzymes NADH and FADH₂

16.8K
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
16.8K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.2K
Overview
80.2K
Fermentation01:29

Fermentation

129.4K
Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
129.4K
The Citric Acid Cycle02:36

The Citric Acid Cycle

162.3K
The citric acid cycle, also known as the Krebs cycle or TCA cycle, consists of several energy-generating reactions that yield one ATP molecule, three NADH molecules, one FADH2 molecule, and two CO2 molecules.
162.3K
Outcomes of Glycolysis01:13

Outcomes of Glycolysis

107.3K
Nearly all the energy used by cells comes from the bonds that make up complex organic compounds. These organic compounds are broken down into simpler molecules, such as glucose. As a result, cells extract energy from glucose over many chemical reactions—a process called cellular respiration.
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate...
107.3K
Energy-releasing Steps of Glycolysis01:28

Energy-releasing Steps of Glycolysis

146.9K
Glycolysis is divided into two phases based on whether energy is utilized or released. While the first phase consumes ATP, the second phase produces energy in the form of ATP and NADH. The energy is released over a sequence of reactions that turns G3P into pyruvate. The energy-releasing phase—steps 6-10 of glycolysis—occurs twice, once for each of the two 3-carbon sugars produced during steps 1-5 of the first phase.
The first energy-releasing step—the 6th step of glycolysis...
146.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unexpected widespread amyloid PET positivity in a patient with CADASIL.

Journal of neurology·2026
Same author

Early and severe masticatory muscle involvement in SOD1-ALS: a case report with biomarker-clinical dissociation.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·2026
Same author

A PbS quantum dot film as a hole transport layer for self-powered AgBiS<sub>2</sub> nanocrystal photodetectors.

Chemical communications (Cambridge, England)·2026
Same author

Artificial intelligence for detecting fetal orofacial clefts and advancing medical education.

Nature communications·2026
Same author

HER2∆16 directs luminal cell identity and estrogen receptor signaling in HER2+ breast cancer.

Nature communications·2026
Same author

Tirofiban for Reduction of TEAR: A Phase 2, Randomized, Open-Label, Blinded End Point, Controlled Trial.

Stroke·2026

Related Experiment Video

Updated: Feb 5, 2026

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts
12:07

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

Published on: July 24, 2012

18.5K

Engineering NADH/NAD

Chen Ling1, Guan-Qing Qiao1, Bo-Wen Shuai1

  • 1Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.

Metabolic Engineering
|September 17, 2018
PubMed
Summary
This summary is machine-generated.

Halomonas bluephagenesis enhances polyhydroxyalkanoate (PHA) production under oxygen limitation by utilizing NADH. Blocking electron transport and adding acetic acid boosts PHA accumulation and controls copolymer composition.

Keywords:
HalomonasNADHNADH/NAD(+)Next generation industrial biotechnologyOxygen limitationPHBPolyhydroxyalkanoatesetf

More Related Videos

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
08:57

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

10.5K
A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
10:28

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

Published on: August 17, 2019

10.4K

Related Experiment Videos

Last Updated: Feb 5, 2026

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts
12:07

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

Published on: July 24, 2012

18.5K
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
08:57

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

10.5K
A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
10:28

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

Published on: August 17, 2019

10.4K

Area of Science:

  • Industrial Biotechnology
  • Microbial Engineering
  • Biochemistry

Background:

  • Halomonas bluephagenesis is a platform strain for next-generation industrial biotechnology (NGIB), excelling in high cell density (HCD) growth and resistance to contamination.
  • It is particularly suitable for producing polyhydroxyalkanoates (PHA) such as poly(3-hydroxybutyrate) (PHB), poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB), and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV).
  • The precise mechanisms governing PHA accumulation under oxygen-limited conditions in this strain remain largely unelucidated.

Purpose of the Study:

  • To investigate the cofactor utilization for poly(3-hydroxybutyrate) (PHB) production in Halomonas bluephagenesis under oxygen limitation.
  • To elucidate the mechanism of enhanced PHA accumulation under oxygen-limited conditions.
  • To engineer H. bluephagenesis for increased PHA yield and controlled monomer composition in PHA copolymers.

Main Methods:

  • Determined that H. bluephagenesis preferentially uses NADH over NADPH for PHB synthesis.
  • Blocked the electron transport pathway involving the etf operon to increase the NADH/NAD+ ratio, thereby enhancing PHA accumulation.
  • Supplemented glucose with acetic acid to optimize redox balance and pyruvate metabolism, leading to increased cell dry weight (CDW) and PHA content.

Main Results:

  • Identified NADH as the primary cofactor for PHB production in H. bluephagenesis, explaining enhanced accumulation under oxygen limitation.
  • Engineered strains achieved up to 90% PHB accumulation in CDW, surpassing the wild type's 84%.
  • Co-cultivation with acetic acid resulted in 94% PHB accumulation and a 22% increase in CDW, with controllable increases in 3HV and 4HB monomer ratios in copolymers.

Conclusions:

  • The study reveals a novel mechanism for enhanced PHA accumulation in H. bluephagenesis under oxygen limitation by utilizing NADH.
  • Systematic modulation of the cellular redox potential in H. bluephagenesis leads to significantly improved PHA yields and tunable monomer ratios in PHA copolymers.
  • This engineered approach offers a cost-effective and scalable strategy for industrial PHA production, reducing energy consumption and process complexity.