Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Well-Defined Rhodium-Gallium Catalytic Sites in a Metal-Organic Framework: Promoter-Controlled Selectivity in Alkyne Semihydrogenation to E-Alkenes.

Journal of the American Chemical Society·2018
Same author

Mechanism of piR-DQ590027/MIR17HG regulating the permeability of glioma conditioned normal BBB.

Journal of experimental & clinical cancer research : CR·2018
Same author

Distinctive distributions and migrations of <sup>239+240</sup>Pu and <sup>241</sup>Am in Chinese forest, grassland and desert soils.

Chemosphere·2018
Same author

Ultrafine Mo-doped SnO

Nanoscale·2018
Same author

[Study on the Migration Resistance of Additives in Disposable Photophobic Infusion Set].

Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation·2018
Same author

Urinary proteomics analysis based on mass spectrometry and identification of therapeutic targets of Shenkangling interventions in rats with adriamycin nephropathy using iTRAQ.

American journal of translational research·2018
Same journal

Simultaneous enantioselective separation of 2-, 3- and 4-chloromethcathinones using supercritical fluid chromatography-tandem mass spectrometry and its application to human oral fluid samples.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Untargeted metabolomics, GC-MS and intelligent sensory analysis reveal wine-thermal induced alterations in chemical, sensory and metabolic bioactivities of Curculigo rhizome.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

A single bioanalytical LC-MS/MS method for the quantification of pelitinib across biological matrices: An insight into in vitro metabolic stability, in vivo pharmacokinetics, and IVIVE.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Multimodal analytical strategy for revealing the related compounds of color variation in Salvia miltiorrhiza after sweating.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Development and validation of a UPLC-MS/MS method for real-time neuropharmacokinetic monitoring of iboga alkaloids in rat brain.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

QC friendly polysorbate quantification in high concentration protein samples.

Journal of pharmaceutical and biomedical analysis·2026
See all related articles

Related Experiment Video

Updated: Feb 5, 2026

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
08:39

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: December 22, 2020

4.7K

From

Wenjing Liu1, Qingqing Song1, Yan Cao1

  • 1Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100029, China.

Journal of Pharmaceutical and Biomedical Analysis
|September 17, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a new metabolomics strategy combining 1H NMR and LC-MS to identify molecular markers for differentiating Cistanche plants. This method successfully distinguished four Cistanche species based on their unique chemical profiles.

Keywords:
(1)H NMR-based non-targeted metabolomicsChemome comparisonCistanche plantsLC–MS-based targeted metabolomicsSpecies differences

More Related Videos

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications
09:50

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Published on: August 20, 2021

4.1K
Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
06:22

Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current

Published on: November 3, 2020

3.8K

Related Experiment Videos

Last Updated: Feb 5, 2026

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
08:39

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: December 22, 2020

4.7K
Fibroblast Derived Human Engineered Connective Tissue for Screening Applications
09:50

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Published on: August 20, 2021

4.1K
Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
06:22

Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current

Published on: November 3, 2020

3.8K

Area of Science:

  • Metabolomics
  • Chemometrics
  • Plant Science

Background:

  • Cistanche species are valuable medicinal and edible plants in arid regions.
  • Accurate differentiation of Cistanche species is crucial due to their economic and ecological importance.
  • Comparative metabolomics offers a powerful approach for species discrimination.

Purpose of the Study:

  • To propose and validate an integrated metabolomics strategy for identifying molecular markers.
  • To compare the chemome profiles of four Cistanche species: C. deserticola, C. salsa, C. tubulosa, and C. sinensis.
  • To establish a reliable method for differentiating closely related Cistanche plants.

Main Methods:

  • Utilized a sequential approach: 1H NMR-based non-targeted metabolomics followed by LC-MS-based targeted metabolomics.
  • 1H NMR identified key signals for species discrimination.
  • LC-MS, including RP-LC, HILIC, and MRM, quantified eighteen potential markers simultaneously.

Main Results:

  • Significant differences in chemome patterns were observed among the four Cistanche species.
  • Echinacoside, acteoside, betaine, mannitol, 6-deoxycatalpol, sucrose, and 8-epi-loganic acid were identified as key discriminatory markers.
  • The integrated strategy successfully differentiated the four species based on these markers.

Conclusions:

  • The proposed strategy 'from 1H NMR-based non-targeted to LC-MS-based targeted metabolomics' is effective for molecular marker discovery.
  • This approach provides a valuable tool for the accurate differentiation of Cistanche plants.
  • The findings contribute to a deeper understanding of Cistanche chemomes and facilitate quality control.