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Angular variables are introduced in rotational dynamics. Comparing the definitions of angular variables with the definitions of linear kinematic variables, it is seen that there is a mapping of the linear variables to the rotational ones. Linear displacement, velocity, and acceleration have their equivalents in rotational motion, which are angular displacement, angular velocity, and angular acceleration. Similar to the rotational variables, a mapping exists from Newton's second law of motion...
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Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening
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Bionic 3D spheroids biosensor chips for high-throughput and dynamic drug screening.

Qian Wu1,2, Xinwei Wei1, Yuxiang Pan1

  • 1Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.

Biomedical Microdevices
|September 17, 2018
PubMed
Summary
This summary is machine-generated.

This study developed a 3D lung cancer spheroid biosensor using interdigitated electrodes for more accurate drug screening. The 3D model demonstrated higher drug resistance and predicted synergistic effects of combined anticarcinogens, improving drug discovery and personalized medicine.

Keywords:
3D cancer spheroidBionic 3D spheroid chipsCancer modelDrug screeningPersonalized medicine

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Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Drug Discovery

Background:

  • Traditional planar cell cultures inadequately mimic in vivo human organ complexity for drug screening.
  • Three-dimensional (3D) spheroids offer a more physiologically relevant model by replicating in vivo tissue architecture and cell-cell interactions.
  • Developing advanced biosensors is crucial for accurate drug efficacy and toxicity evaluation.

Purpose of the Study:

  • To engineer a 3D lung cancer spheroid-based biosensor for evaluating drug efficacy.
  • To compare drug resistance between 3D spheroids and traditional planar cell models.
  • To assess the potential of 3D spheroids in personalized medicine and drug discovery.

Main Methods:

  • Utilized lung carcinoma cell lines (A549, H1299, H460) to create 3D lung cancer spheroids.
  • Employed interdigitated electrodes for electric impedance sensing to quantitatively evaluate anticarcinogen inhibition.
  • Applied a connected multiwell interdigitated electrode array for multi-organoid spheroid testing.

Main Results:

  • 3D lung cancer spheroids exhibited significantly higher drug resistance compared to planar cell models.
  • Electric impedance sensing effectively quantified anticarcinogen effects on various 3D lung cancer spheroid models.
  • Combination therapy of cisplatin and etoposide showed a synergistic effect with enhanced drug response in A549 spheroids.

Conclusions:

  • The 3D cancer spheroid biosensor provides a more predictive platform for drug discovery and personalized medicine screening.
  • This advanced biosensor technology holds significant promise for applications in pharmacy and clinical medicine.
  • 3D spheroid models represent a crucial advancement over 2D cultures for in vitro drug evaluation.