[Activation of ras and myc proto-oncogenes in human breast carcinoma and neuroblastoma]

Related Experiment Video

Updated: Aug 9, 2026

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
07:13

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Published on: March 27, 2014

P G Kniazev, R Schafer, K Willecke

    Molekuliarnaia Biologiia
    |September 1, 1986
    PubMed

    Insights

    Human breast carcinoma and neuroblastoma DNA can transform NIH 3T3 cells, revealing amplified oncogenes. This suggests ras and myc gene cooperation drives tumor development.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Genetics

    Context:

    • Investigates the role of oncogenes in human cancers.
    • Utilizes DNA-mediated gene transfer and blot hybridization techniques.
    • Examines DNA from human breast carcinoma (SK-BR-3) and neuroblastoma (LA-N-1) cell lines.

    Purpose:

    • To determine if DNA from specific human tumors can induce NIH 3T3 cell transformation.
    • To identify oncogenes involved in the transformation process.
    • To explore the potential cooperation between ras and myc oncogenes in tumorigenesis.

    Summary:

    • DNA from SK-BR-3 and LA-N-1 cell lines induced NIH 3T3 cell transformation.
    • Analysis revealed amplification of nucleotide sequences homologous to the v-myc oncogene.
    • Amplified myc sequences corresponded to c-myc and N-myc loci, indicating active Ha-ras and altered myc oncogenes coexist in these tumors.

    Impact:

    • Demonstrates that oncogene activation is a key event in tumor development.
    • Suggests a multi-step mechanism involving ras and myc gene cooperation in breast carcinoma and neuroblastoma.
    • Provides insights into the molecular basis of these complex diseases.

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