Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

NaYF

Zhenli Qiu1, Jian Shu1, Dianping Tang1

  • 1Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry , Fuzhou University , Fuzhou , Fujian 350116 , People's Republic of China.

Analytical Chemistry
|September 18, 2018
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emerging trends in amino acid detection: wearable devices and machine learning-assisted signal processing.

Analytical methods : advancing methods and applications·2026
Same author

Overriding <i>n</i>-Type Surface Depletion via Ferroelectric Pumping for Self-Powered Cathodic Photoelectrochemical Immunoassay.

Analytical chemistry·2026
Same author

Mn-Fe bimetallic oxide-mediated photothermal immunoassay for portable screening of carcinoembryonic antigen.

Analytical and bioanalytical chemistry·2026
Same author

MultiGO++: Monocular 3D Clothed Human Reconstruction via Geometry-Texture Collaboration.

IEEE transactions on visualization and computer graphics·2026
Same author

A CRISPR/Cas12a-MXene Nanozyme Platform for Universal Detection of Trace DNA.

ACS sensors·2026
Same author

Lectin-based glycoproteomic profiling reveals salivary N-glycan changes recognized by Datura stramonium agglutinin in hepatitis B-related cirrhosis.

Carbohydrate research·2026

A novel aptasensor uses near-infrared light to detect carcinoembryonic antigen (CEA). This photoelectrochemical method, utilizing upconversion nanoparticles and hybridization chain reaction, offers high sensitivity for cancer biomarker screening.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Carcinoembryonic antigen (CEA) is a significant biomarker for various cancers.
  • Sensitive and specific detection of CEA is crucial for early diagnosis and monitoring.
  • Existing detection methods often require complex procedures or lack sufficient sensitivity.

Purpose of the Study:

  • To develop a highly sensitive and specific photoelectrochemical aptasensor for CEA detection.
  • To leverage near-infrared (NIR) light and upconversion nanoparticles (UCNs) for enhanced signal transduction.
  • To utilize a hybridization chain reaction (HCR) for signal amplification in the aptasensor.

Main Methods:

  • Construction of a photoelectrochemical aptasensor using NaYF4:Yb,Er upconversion nanoparticles (UCNs) and in situ formed Ag2S nanoparticles.

Related Experiment Videos

  • NIR light activation of UCNs to convert light into detectable wavelengths for semiconductor harvesting.
  • Signal amplification via a sandwich-type assay involving CEA aptamers and hybridization chain reaction (HCR) with Ag+ chelation and subsequent Ag2S formation.

    • Main Results:

    • The developed aptasensor demonstrated high sensitivity for CEA detection within a dynamic linear range of 0.005-5.0 ng mL-1.
    • Achieved a low limit of detection of 1.9 pg mL-1 for CEA.
    • Exhibited good precision, high specificity, and reliable performance in human serum samples compared to ELISA kits.

    Conclusions:

    • The NaYF4:Yb,Er-based NIR light-responsive PEC aptasensing system provides a novel and efficient platform for sensitive CEA detection.
    • This approach offers a promising strategy for detecting disease-related biomarkers using nucleic acid amplification.
    • The developed aptasensor has potential applications in clinical diagnostics and cancer research.