A highly sensitive peptide-based biosensor using NiCo

Area of Science:

• Electrochemistry
• Biosensor Technology
• Nanomaterials

Background:

• Trypsin is a key biomarker in various physiological processes and diseases.
• Development of sensitive and selective biosensors is crucial for early disease diagnosis.
• Electrochemical biosensors offer advantages like simplicity, cost-effectiveness, and high sensitivity.

Purpose of the Study:

• To develop a simple and sensitive electrochemical biosensor for trypsin detection.
• To utilize the specific recognition between trypsin and peptides for signal generation.
• To explore the potential of NiCo2O4-PAMAM nanocomposite and g-C3N4 labeled peptides in biosensor design.

Main Methods:

• Fabrication of an electrode modified with NiCo2O4-PAMAM nanocomposite for signal amplification.
• Immobilization of graphene carbon nitride (g-C3N4) labeled peptides onto the modified electrode.
• Electrochemical detection of trypsin based on the signal change upon peptide cleavage.
• Characterization of the electrochemical performance, including linear range and detection limit.

Main Results:

• The NiCo2O4-PAMAM nanocomposite enhanced the electrochemical signal due to its conductivity and activity.
• A decrease in signal was observed after immobilizing peptide composites, followed by signal recovery upon trypsin addition.
• The developed biosensor exhibited a linear detection range from 10⁻¹⁰ to 10⁻⁴ mg/mL.
• An ultralow detection limit of 10⁻¹⁰ mg/mL was achieved, demonstrating high sensitivity.

Conclusions:

• The developed peptide-based electrochemical biosensor enables sensitive and selective detection of trypsin.
• The biosensor shows excellent stability, reproducibility, and accuracy, making it suitable for clinical applications.
• This approach holds promise for disease diagnosis and monitoring through accurate trypsin quantification.