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Updated: Feb 5, 2026

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
Published on: August 15, 2018
The application of cellulose acetate replication sheets in enamel wear investigations
Manhal Ijbara1, Kanae Wada1, Junichiro Wada2
1Pediatric Dentistry Department, Division of Oral Restitution, Graduate School, Tokyo Medical and Dental University (TMDU).
Abstract:
Surface replication is a nondestructive evaluation technique applied in examining surfaces' cracks/wear. This is quite valuable when the surface of interest cannot be further manipulated by cutting or polishing to fit directly under a microscope. Replication by acetate sheets is one of the techniques applied in industrial, metallographic and anthropological wear/examination. Enamel is the outermost protective layer of human teeth and is subjected to mechanical stresses due to the masticatory function; thus, wear is inevitable in human teeth. This relatively inert hard tissue has been reported to possess some properties of both metals and glasses due to its unique delicate microstructure. This study investigated the applicability of replication sheets in registering wear induced features on human enamel surfaces. The sheets replicated wear features successfully with compatibility to use with multiple microscopes. Acetate sheets have a potential in enamel wear replication.
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