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Updated: Feb 5, 2026

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
Published on: April 8, 2017
Differential expression analysis of transcriptome data of
Huafang Shi1, Kiantra Butler1, Christian Tschudi1
1Department of Epidemiology of Microbial Diseases, Yale School of Public Health, 60 College Street, New Haven, CT 06520, USA.
Abstract:
We used an in vitro system based on the inducible expression of the RNA binding protein 6 (RBP6) to monitor transcriptome changes during the differentiation of Trypanosoma brucei from non-infectious procyclics to infectious metacyclics and from metacyclics to bloodstream forms. This data file describes the bioinformatics analysis of 20 distinct RNA-Seq samples, with four biological replicates each, highlighting differential transcript abundance. Additional functional annotation analysis using Gene Ontology is also presented. Complete raw data files were deposited at the NCBI Sequence Read Archive - SRA at http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi with accession numbers: SRP153824, SRP153562, and SRP152737.
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