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Related Experiment Videos

Receptors for IgM on certain human B lymphocytes.

M Ferrarini, T Hoffman, S M Fu

    Journal of Immunology (Baltimore, Md. : 1950)
    |October 1, 1977
    PubMed
    Summary

    Researchers identified a novel receptor for Immunoglobulin M (IgM) on human B lymphocytes using a rosette technique. This IgM receptor is expressed on normal and malignant B cells, particularly after culture in specific media.

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    CLASSIFICATION OF MYELOMA PROTEINS, BENCE JONES PROTEINS, AND MACROGLOBULINS INTO TWO GROUPS ON THE BASIS OF COMMON ANTIGENIC CHARACTERS.

    The Journal of experimental medicine·2009

    Area of Science:

    • Immunology
    • Cell Biology
    • Hematology

    Background:

    • B lymphocytes play a crucial role in the adaptive immune system.
    • Surface receptors on lymphocytes are key to immune cell function and communication.
    • Previous research identified receptors for other immunoglobulins on various immune cells.

    Purpose of the Study:

    • To demonstrate and characterize a specific receptor for Immunoglobulin M (IgM) on human B lymphocytes.
    • To investigate the expression patterns and specificity of this newly identified IgM receptor.
    • To explore the presence of IgM receptors on both normal and malignant B cells.

    Main Methods:

    • Rosette technique using ox erythrocytes coated with rabbit IgM antibody (EAM).
    • Inhibition experiments with purified human immunoglobulins (IgM, IgG) and IgM fragments.

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  • Analysis of cell surface markers including Ia-like antigens and surface immunoglobulin.
  • Culturing of lymphocytes in IgM-free media to assess receptor expression.
  • Examination of various human lymphoid tissues and chronic lymphocytic leukemia (CLL) cells.

    • Main Results:

    • A specific receptor for IgM was demonstrated on human B lymphocytes via EAM rosettes.
    • The IgM receptor specifically binds to the Fc portion of the IgM molecule.
    • Receptors were not detected on freshly isolated B cells but were expressed after overnight culture in IgM-free media.
    • IgM receptors were found on a limited percentage of circulating and non-circulating B cells.
    • A majority of chronic lymphocytic leukemia (CLL) B cells expressed these IgM receptors.

    Conclusions:

    • Human B lymphocytes possess a distinct receptor for IgM, primarily binding to its Fc region.
    • The expression of IgM receptors is regulated by cell culture conditions and is present on both normal and malignant B cells.
    • This finding contributes to understanding B cell heterogeneity and immune regulation in B cell malignancies like CLL.