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Author Spotlight: Deciphering the Mysteries of Skeletal Muscle Fiber Types Using the MyDoBID Technique
Published on: September 22, 2023
Total Biosynthesis of Brassicicenes: Identification of a Key Enzyme for Skeletal Diversification
Akihiro Tazawa1, Ying Ye1, Taro Ozaki1
1Department of Chemistry, Faculty of Science , Hokkaido University , Sapporo 060-0810 , Japan.
Abstract:
The biosynthetic pathway of brassicicenes, derived from the phytopathogen Pseudocercospora fijiensis, was fully reconstituted. Heterologous expression of the eight genes highly expressed in infected leaf tissues generated a new brassicicene derivative as a final product. Together with the characterization of P450 from Alternaria brassicicola, a late stage of the biosynthetic pathway, which generates remarkable structural diversity, has been proposed. Notably, a unique P450 that converts 3 to the structurally distinct 4 and 6 was identified.
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