Related Experiment Video

Updated: Aug 14, 2026

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
07:09

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

Purification and characterization of human interleukin-1 beta expressed in recombinant Escherichia coli

P Wingfield, M Payton, J Tavernier

    European Journal of Biochemistry
    |November 3, 1986
    PubMed

    More Related Videos

    Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli07:35

    Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

    Published on: June 9, 2014

    Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation12:55

    Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

    Published on: September 29, 2019

    Related Concept Videos

    Production of Pharmaceuticals01:30

    Production of Pharmaceuticals

    Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...