Related Experiment Video
Updated: Feb 5, 2026
09:30
Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
Published on: August 17, 2022
3.6K
Assessing the Value of Next-Generation Sequencing Tests in a Dynamic Environment
Howard A Burris1, Leonard B Saltz1, Peter P Yu1
1From Sarah Cannon, Nashville, TN; Memorial Sloan Kettering Cancer Center, New York, NY; Hartford HealthCare Cancer Institute, Hartford, CT, Memorial Sloan Kettering Cancer Center, New York, NY.
Summary
Next-generation sequencing (NGS) offers affordable cancer genomic testing. Understanding NGS test variations and clinical utility is crucial for oncologists navigating evolving therapies and healthcare policies.
Area of Science:
- Oncology
- Genomics
- Health Policy
Background:
- Next-generation sequencing (NGS) technology has reduced cancer genomic alteration testing costs.
- Commercial availability of NGS tests is increasing across diagnostic laboratories.
- Variations exist in NGS methodologies, genomic alteration coverage, and clinical annotation.
Purpose of the Study:
- To review principles and practices for NGS testing in oncology.
- To address the dynamic landscape of cancer biology, therapies, and healthcare policy.
- To inform oncologists about the value of NGS testing in clinical decision-making.
Main Methods:
- Review of current NGS technologies and their application in cancer testing.
- Analysis of factors influencing the value of NGS tests (e.g., clinical trials, routine care).
- Examination of the evolving therapeutic landscape and reimbursement policies.
Main Results:
- NGS test value is context-dependent, influenced by clinical trial support or routine care integration.
- The rapid evolution of investigational drugs and FDA-approved therapeutics impacts NGS test utility.
- Reimbursement policies, such as those from CMS, are adapting to the defined value of NGS testing.
Conclusions:
- Oncologist knowledge of NGS testing value is essential for patient care and clinical decisions.
- Effective utilization of NGS requires understanding its technical, clinical, and policy dimensions.
- Navigating the confluence of technology, cancer biology, and health policy is key for optimal NGS implementation.
Related Concept Videos
03:00Next-generation Sequencing
98.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
98.6K
02:02Cis-regulatory Sequences
11.8K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.8K
01:20Gene-Environment Interactions
1.2K
Gene expression is a dynamic process that is significantly influenced by environmental factors. This interaction underlies the complex nature of biological development and the phenotypic differences observed among individuals, even among those with identical genetic makeups. Factors such as radiation, temperature, behavior, nutrition, and stress play pivotal roles in determining how genes are expressed. The concept of the reaction range is central to understanding this interaction. It posits...
1.2K
02:20Dynamic Equilibrium
62.7K
A reversible chemical reaction represents a chemical process that proceeds in both forward (left to right) and reverse (right to left) directions. When the rates of the forward and reverse reactions are equal, the concentrations of the reactant and product species remain constant over time and the system is at equilibrium. A special double arrow is used to emphasize the reversible nature of the reaction. The relative concentrations of reactants and products in equilibrium systems vary greatly;...
62.7K
01:29Sequences
278
Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
278
01:57Sanger Sequencing
774.7K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
774.7K