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Updated: Feb 5, 2026

Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish
Published on: February 22, 2019
New Molecular Technologies for Minimal Residual Disease Evaluation in B-Cell Lymphoid Malignancies
Irene Dogliotti1,2, Daniela Drandi3, Elisa Genuardi4
1Hematology Division, Department of Molecular Biotechnologies and Health Sciences, University of Torino, 10123 Torino, Italy. irenedogl@hotmail.com.
Abstract:
The clearance of malignant clonal cells significantly correlates with clinical outcomes in many hematologic malignancies. Accurate and high throughput tools for minimal residual disease (MRD) detection are needed to overcome some drawbacks of standard molecular techniques; such novel tools have allowed for higher sensitivity analyses and more precise stratification of patients, based on molecular response to therapy. In this review, we depict the recently introduced digital PCR and next-generation sequencing technologies, describing their current application for MRD monitoring in lymphoproliferative disorders. Moreover, we illustrate the feasibility of these new technologies to test less invasive and more patient-friendly tissues sources, such as "liquid biopsy".
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