Sirt1 protects neural stem cells from apoptosis by decreasing acetylation of histone 3K9

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Chongdong Jian1, Cuihua Zou1, Ning Xu2

  • 1Youjiang Medical University for Nationalities, Baise, Guangxi 533000, People's Republic of China.

Stem Cells and Cloning : Advances and Applications
|September 21, 2018
PubMed
Abstract

Insights

Sirtuin 1 (Sirt1) protects neural stem cells (NSCs) from apoptosis. Overexpressing Sirt1 reduced caspase-3 cleavage and histone acetylation, suggesting a protective mechanism.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Neural stem cells (NSCs) are crucial for brain development and repair.
  • Apoptosis, or programmed cell death, is a critical process that can impact NSC function.
  • Sirtuin 1 (Sirt1) is a protein deacetylase implicated in various cellular processes, including stress resistance and aging.

Purpose of the Study:

  • To investigate the protective role of Sirtuin 1 (Sirt1) in neural stem cells (NSCs).
  • To elucidate the underlying molecular mechanism by which Sirt1 influences NSC apoptosis.

Main Methods:

  • Rat neural stem cells (NSCs) were transfected to overexpress Sirtuin 1 (Sirt1).
  • Apoptosis was induced using camptothecin, and its effect on Sirt1-overexpressing NSCs was assessed.
  • Western blotting was employed to measure levels of Sirt1, cleaved caspase-3, and acetylated histone 3 lysine 9 (H3K9).

Main Results:

  • Overexpression of Sirtuin 1 (Sirt1) in neural stem cells (NSCs) led to a reduction in cleaved caspase-3.
  • Sirt1 overexpression also decreased the acetylation of histone 3 lysine 9 (H3K9).

Conclusions:

  • Sirtuin 1 (Sirt1) demonstrates a protective effect against apoptosis in neural stem cells (NSCs).
  • The mechanism involves the downregulation of histone 3 lysine 9 (H3K9) acetylation, suggesting Sirt1's role as a key regulator in NSC survival.
Keywords:
Sirt1acetylated histone 3K9apoptosiscaspase-3neural stem cells

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