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Related Concept Videos

Phases of Wound Repair01:28

Phases of Wound Repair

Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...

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Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres
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Alginate-Based Hydrogels with Amniotic Membrane Stem Cells for Wound Dressing Application.

Nurul Fitriani1,2, Gofarana Wilar3, Angga Cipta Narsa2

  • 1Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, 45363, Indonesia.

Stem Cells and Cloning : Advances and Applications
|January 16, 2025
PubMed
Summary

This study developed a novel amniotic membrane-derived stem cell (AMSC) alginate hydrogel (AMSC/Alg-H) for in vitro wound healing. The AMSC/Alg-H biomaterial demonstrated 100% wound closure in 24 hours, significantly enhancing cell proliferation and migration.

Keywords:
AMSCalginatehydrogelwound healing

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Stem Cell Biology

Background:

  • Chronic wounds pose a significant clinical challenge, driving the need for advanced regenerative therapies.
  • Amniotic membrane-derived stem cells (AMSCs) show promise for tissue repair.
  • Alginate hydrogels offer a versatile scaffold for cell delivery and tissue engineering.

Purpose of the Study:

  • To develop and evaluate an innovative biomaterial for in vitro wound healing.
  • To prepare an AMSC-loaded alginate hydrogel (AMSC/Alg-H) cross-linked with calcium chloride.
  • To assess the in vitro effectiveness of AMSC/Alg-H for promoting wound closure.

Main Methods:

  • Formulation of AMSC/Alg-H hydrogel by combining AMSCs, sodium alginate, and calcium chloride.
  • Characterization using Scanning Electron Microscopy (SEM), FTIR, and Differential Scanning Calorimetry (DSC).
  • Evaluation of cytotoxicity and in vitro wound healing capacity via cell scratch assay and TGF-β1 measurement.

Main Results:

  • Successful formulation and characterization of AMSC/Alg-H hydrogel, confirming MSC encapsulation and crosslinking.
  • AMSC/Alg-H exhibited no significant cytotoxicity to HaCaT cells.
  • Achieved 100% wound closure within 24 hours, surpassing controls and enhancing cell proliferation and migration.

Conclusions:

  • AMSC/Alg-H demonstrates significant potential as an advanced regenerative therapy for in vitro wound healing.
  • The biomaterial effectively maintains AMSC viability and promotes tissue-like structure formation.
  • This hydrogel represents a promising strategy for accelerating chronic wound closure.