Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

13.6K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
13.6K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.4K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.4K
Atomic Force Microscopy01:08

Atomic Force Microscopy

4.5K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.5K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

16.4K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
16.4K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.1K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

14.7K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transient Erythema Elevatum Diutinum Associated With HIV Viremia.

Cureus·2023
Same author

Association of Tumor Characteristics With Insurance Type Among Patients Undergoing Mohs Micrographic Surgery for Nonmelanoma Skin Cancer.

JAMA dermatology·2022
Same author

Erythematous papules and pustules on the nose.

Cutis·2019
Same author

Management of a neonate with diffuse cutaneous mastocytosis: Case report and literature review.

Pediatric dermatology·2019
Same author

Disseminated Syringomas of the Upper Extremities in a Young Woman.

Cureus·2019
Same author

Allergy testing in dermatology and beyond.

Cutis·2018
Same journal

Black Dots on the Scalp of a Child.

Cutis·2026
Same journal

Psoriasis and Obesity: A Clinical Review of the Bidirectional Link and Management Implications.

Cutis·2026
Same journal

Adalimumab in Lichen Planus: A Narrative Review of Treatment and Paradoxical Reactions.

Cutis·2026
Same journal

The Future of Psoriasis Care.

Cutis·2026
Same journal

Evaluating GPT-4o for Automated Classification of Skin Lesions Using the HAM10000 Dataset.

Cutis·2026
Same journal

Metastatic Primary Extramammary Paget Disease: A Case Series.

Cutis·2026
See all related articles

Related Experiment Video

Updated: Feb 5, 2026

A Bedside, Single Burr Hole Approach to Multimodality Monitoring in Severe Brain Injury
06:18

A Bedside, Single Burr Hole Approach to Multimodality Monitoring in Severe Brain Injury

Published on: March 26, 2019

9.6K

Bedside microscopy for the beginner.

Ashley D Lundgren1

  • 1Division of Dermatology, University of Texas Dell Medical School, Austin, USA.

Cutis
|September 21, 2018
PubMed
Summary
This summary is machine-generated.

This guide helps new microscopists identify skin lesions for bedside testing. It details specimen preparation, analysis, and interpretation for outpatient microscopic evaluation.

More Related Videos

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
12:52

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Published on: October 13, 2014

17.1K
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

10.2K

Related Experiment Videos

Last Updated: Feb 5, 2026

A Bedside, Single Burr Hole Approach to Multimodality Monitoring in Severe Brain Injury
06:18

A Bedside, Single Burr Hole Approach to Multimodality Monitoring in Severe Brain Injury

Published on: March 26, 2019

9.6K
Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
12:52

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Published on: October 13, 2014

17.1K
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

10.2K

Area of Science:

  • Dermatology
  • Microscopy
  • Pathology

Background:

  • Novice microscopists face challenges in selecting appropriate lesions for bedside testing.
  • Difficulties arise in specimen preparation and interpretation for microscopic analysis.

Purpose of the Study:

  • To guide clinicians in identifying primary skin lesions suitable for outpatient microscopic evaluation.
  • To provide detailed instructions on specimen collection and preparation.
  • To offer basic interpretation guidelines for microscopic findings.

Main Methods:

  • Review of dermatological conditions requiring microscopic examination.
  • Standardized protocols for bedside specimen collection (e.g., skin scraping, biopsy).
  • Microscopic analysis techniques and common findings.

Main Results:

  • Identification of key lesion characteristics prompting microscopic testing.
  • Step-by-step procedures for sample acquisition and slide preparation.
  • Common microscopic findings and their diagnostic significance.

Conclusions:

  • Empowering novice microscopists with essential skills for outpatient dermatological diagnosis.
  • Improving accuracy and efficiency in bedside microscopic evaluations.
  • Facilitating timely and appropriate patient management through effective lesion assessment.