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Updated: Feb 5, 2026
Synthesis of a Water-soluble Metal–Organic Complex Array
Published on: October 8, 2016
Synthesis and Application of Water-Soluble Oxazine Dyes for Detection of PHAs-Producing Bacteria
Qing-Hao Liu1, Jin-Chun Guo2, Hong-Yu Lu3
1School of Chemical Engineering and Technology, North University of China, Taiyuan, 030051, China. liuqinghao@nuc.edu.cn.
Abstract:
Derivatives of oxazine dyes were synthesized on mulitigram scales via efficient synthetic strategies. One practical route was selected to prepare compounds 6, 9 and 10, especially water-soluble compound 6 was obtained in better yield than reported, and compound 10 was insoluble in aqueous media in absence of phenolic-OH. Compounds 3 and 9 were found to be clear pH-dependent between pH = 4.0 and 10.0, and could be used as acid-base indicators to measure intracellular pH. Compounds 6, 9, 10 all have carboxylic acid functionalities, which could be activated and used to conjugate the dyes to biomolecules. In addition, compounds 6 and 9 with good solubility in aqueous media were used to develop a simple, quick, safe, highly sensitive staining method to detect PHAs-producing bacteria on heat-fixed smears, which was confirmed by fluorescence images of PHAs granules of bacteria.
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