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Updated: Feb 5, 2026

Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System
Published on: October 10, 2014
CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors
David L Hacker1, Laurence Durrer2, Soraya Quinche2
1Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. david.hacker@epfl.ch.
Abstract:
Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the two most popular mammalian hosts for the production of recombinant proteins. In this chapter the suspension cultivation and transfection of these cells in small-scale disposable bioreactors is described. The TubeSpin bioreactor 50 and TubeSpin bioreactor 600 are designed for the cultivation of suspension cells by orbital shaking and have maximum working volumes of about 15 mL and 400 mL, respectively.
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