Related Experiment Video

Updated: Feb 4, 2026

Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression
07:53

Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression

Published on: March 17, 2020

7.7K

The circular RNA circ-ITCH suppresses ovarian carcinoma progression through targeting miR-145/RASA1 signaling

Jinghui Hu1, Li Wang2, Jiming Chen3

  • 1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou, China; Department of Gynecology, The First Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.

Biochemical and Biophysical Research Communications
|September 24, 2018
PubMed

Insights

Circular RNA (circRNA) circ-ITCH is downregulated in ovarian cancer (OC), suppressing tumor growth and progression. This study reveals circ-ITCH as a novel tumor suppressor in OC via the circ-ITCH-miR-145-RASA1 axis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Ovarian cancer (OC) is a leading cause of gynecological cancer death.
  • Circular RNAs (circRNAs) are emerging as key regulators in various cellular processes.
  • circ-ITCH, a known tumor suppressor in other cancers, has not been studied in OC.

Purpose of the Study:

  • To investigate the role of circ-ITCH in ovarian cancer.
  • To elucidate the underlying molecular mechanisms of circ-ITCH in OC progression.

Main Methods:

  • Analysis of circ-ITCH expression in OC tissues and cells.
  • Functional assays including CCK8, cell cycle, wound healing, and invasion assays.
  • In vivo tumorigenesis studies in xenograft NOD mice.
  • Mechanistic studies involving RNA immunoprecipitation and luciferase reporter assays to confirm the circ-ITCH-miR-145-RASA1 axis.

Main Results:

  • circ-ITCH was broadly downregulated in OC tissues and cells, correlating with poor patient prognosis.
  • Overexpression of circ-ITCH inhibited OC cell viability, motility, and tumorigenesis in vitro and in vivo.
  • circ-ITCH functions as a competing endogenous RNA (ceRNA) by sponging miR-145, thereby increasing RASA1 levels.
  • The circ-ITCH-miR-145-RASA1 axis was confirmed to inhibit OC cell malignant progression.

Conclusions:

  • circ-ITCH exhibits a novel tumor-suppressive role in ovarian cancer.
  • The circ-ITCH-miR-145-RASA1 axis represents a potential therapeutic target for OC treatment.
Keywords:
Circular RNA (circRNA)Ovarian cancerRASA1miR-145

More Related Videos

Identification of Circular RNAs using RNA Sequencing08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.8K
Use of Alu Element Containing Minigenes to Analyze Circular RNAs13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.7K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.8K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.1K
Deformation in a Circular Shaft01:10

Deformation in a Circular Shaft

One of the distinctive characteristics of circular shafts is their ability to maintain their cross-sectional integrity under torsion. In other words, each cross-section continues to exist as a flat, unaltered entity, simply rotating like a solid, rigid slab. To understand the distribution of shearing stress within such a shaft, consider a cylindrical section inside this circular shaft. This section has a length of L and a radius of R, with one end fixed. The radius of the cylindrical section is...
924
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
32.8K
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.4K
What is Cell Signaling?02:03

What is Cell Signaling?

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
130.9K