Related Experiment Video

Updated: Feb 4, 2026

Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
09:45

Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines

Published on: August 21, 2019

7.5K

Silencing of FTS increases radiosensitivity by blocking radiation-induced Notch1 activation and spheroid formation in

D S Prabakaran1, Sridhar Muthusami2, T Sivaraman3

  • 1Department of Radiation Oncology, Chungbuk National University College of Medicine, Cheongju 28644, Republic of Korea.

International Journal of Biological Macromolecules
|September 24, 2018
PubMed

Insights

Fused Toes Homolog (FTS) regulates cancer stem cell (CSC) activation and radioresistance in cervical cancer by influencing Notch signaling. Targeting FTS may enhance radiosensitivity, offering a new therapeutic strategy for cervical cancer patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Cancer stem cells (CSCs) drive tumor radioresistance and recurrence.
  • Notch signaling is crucial for CSC maintenance in various cancers, including cervical cancer.
  • Fused Toes Homolog (FTS) has been linked to radioresistance in cervical cancer.

Purpose of the Study:

  • To investigate the regulatory role of FTS in Notch signaling and CSC maintenance following irradiation of cervical cancer cells.
  • To determine if FTS influences the radiation-induced activation of CSCs.

Main Methods:

  • Irradiation of cervical cancer cells.
  • Quantitative analysis of Notch signaling molecules (Notch1, 2, 3, cleaved Notch1, Hes1) and CSC markers (Nanog, Oct4A, Sox2).
  • FTS silencing using RNA interference.
  • Spheroid formation assays.
  • Immunoprecipitation and in silico structural analysis to assess FTS-Notch1 interaction.

Main Results:

  • Irradiation increased Notch signaling components and spheroid formation, indicating CSC activation.
  • FTS silencing abrogated radiation-induced increases in Notch signaling and spheroid formation.
  • FTS was shown to bind Notch1 and Hes1.
  • Combined FTS silencing and radiation significantly reduced spheroid formation and CSC marker expression.

Conclusions:

  • FTS plays a key role in regulating irradiation-induced Notch signaling and CSC activation in cervical cancer.
  • FTS represents a potential therapeutic target to enhance cervical cancer radiosensitivity.
Keywords:
Cancer stem cellsCervical cancerFused Toes HomologNotchRadiation

More Related Videos

Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates11:24

Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates

Published on: March 7, 2017

7.4K
Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

29.4K

Related Concept Videos

Biological Effects of Radiation02:59

Biological Effects of Radiation

All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
18.0K
Increasing Function01:18

Increasing Function

An increasing function exhibits a rise in output values as input values increase. This behavior is depicted graphically as a curve or line that slopes upward from left to right. Such a function satisfies the condition that if x1 < x2, then f(x1) < f(x2), indicating that the function values grow with increasing inputs. This concept is fundamental in understanding growth trends across various domains, such as population dynamics, financial investments, or resource consumption.The...
397
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
7.0K
Radiation: Applications01:17

Radiation: Applications

The average temperature of Earth is the subject of much current discussion. Earth is in radiative contact with both the Sun and dark space; it receives almost all its energy from the radiation of the Sun and reflects some of it into outer space. Dark space is very cold, about 3 K, so Earth radiates energy into it. For instance, heat transfer occurs from soil and grasses, the rate of which can be so rapid that frost can occur on clear summer evenings, even in warm latitudes.
The average...
1.8K
Absorption of Radiation01:05

Absorption of Radiation

The rate of heat transfer by emitted radiation is described by the Stefan-Boltzmann law of radiation:
1.3K
Increased Body Temperature01:25

Increased Body Temperature

A body temperature above  38°C  (100.4 °F) is known as fever or pyrexia, and a person with fever is termed 'febrile.' Typically, the hypothalamus, a part of the brain that acts as the body's thermostat, regulates body temperature through a thermoregulatory setpoint. It receives signals from cold and warm thermal receptors throughout the body and adjusts the body's temperature accordingly. Fever occurs when this hypothalamic setpoint is altered, usually in...
7.5K