Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Structural Joints: Synovial Joints01:16

Structural Joints: Synovial Joints

6.9K
Synovial joints are the most common type of joint in the body. A key structural characteristic for a synovial joint is the presence of a joint cavity. This fluid-filled space is where the articulating surfaces of the bones contact each other. Also, unlike fibrous or cartilaginous joints, the articulating bone surfaces at a synovial joint are not directly connected to each other with fibrous connective tissue or cartilage. This gives the bones of a synovial joint the ability to move smoothly...
6.9K
Structural Joints: Fibrous Joints01:03

Structural Joints: Fibrous Joints

3.8K
Fibrous joints are a type of joint where the bones are connected by fibrous connective tissue. These joints provide stability and minimal to no movement between the articulating bones. There are three types of fibrous joints.
Suture
All the bones of the skull, except for the mandible, are joined to each other by a fibrous joint called a suture. The fibrous connective tissue found at a suture strongly unites the adjacent skull bones and thus helps to protect the brain and form the face. In...
3.8K
Structural Joints: Cartilaginous Joints01:17

Structural Joints: Cartilaginous Joints

4.0K
As the name indicates, at a cartilaginous joint, the adjacent bones are united by cartilage, a tough but flexible type of connective tissue. Unlike synovial joints, these types of joints lack a joint cavity and involve bones joined together by either hyaline cartilage or fibrocartilage.
There are two types of cartilaginous joints:
Synchondrosis
A synchondrosis ("joined by cartilage") is a cartilaginous joint where bones are connected by hyaline cartilage. Synchondrosis may be temporary...
4.0K
Joints01:26

Joints

35.8K
Joints, also called articulations or articular surfaces, are points at which ligaments or other tissues connect adjacent bones. Joints permit movement and stability, and can be classified based on their structure or function.
Structural joint classifications are based on the material that makes up the joint as well as whether or not the joint contains a space between the bones. Joints are structurally classified as fibrous, cartilaginous, or synovial.
Fibrous Joints Are Immovable
The bones of a...
35.8K
Method of Joints01:30

Method of Joints

1.3K
The method of joints is a commonly used technique to analyze the forces in structural trusses. The method is based on the principle of equilibrium, which assumes that the truss members are connected by frictionless pins. The forces at each joint can be determined by considering the equilibrium of the forces acting on that joint.
Since plane truss members are in the same plane, each joint is subjected to a coplanar and concurrent force system. To apply the method of joints, the first step is to...
1.3K
Introduction to Joints00:58

Introduction to Joints

4.8K
The adult human body usually has 206 bones, and except for the hyoid bone in the neck, each bone is connected to at least one other bone. Joints are the location where bones come together. Many joints allow for movement between the bones. At these joints, the articulating surfaces of the adjacent bones can move smoothly against each other. However, the bones of other joints may be joined by connective tissue or cartilage. These joints are designed for stability and provide little or no...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MRGPRX2 D184 engagement by food and pollen allergens: a computational hypothesis for IgE-independent mast-cell activation.

International archives of allergy and immunology·2026
Same author

A Sensor-to-Initiation Proteome Architecture Governing Regeneration Commitment in <i>Turritopsis Species</i>.

Journal of proteome research·2026
Same author

Transcriptome-Based Evaluation of Hydrogen Gas Effects for Preventing UVA-Induced Photoaging Using an Artificial Skin Model.

Geriatrics & gerontology international·2026
Same author

MRGPRX2-Mediated Mast Cell Degranulation by Monomethyl Methacrylate: Unveiling a Pathway in Bone Cement Implantation Syndrome.

Clinical and experimental pharmacology & physiology·2025
Same author

Time-sensitive effects of quercetin on rat basophilic leukemia (RBL-2H3) cell responsiveness and intracellular signaling.

PloS one·2025
Same author

Denervation-Induced Sarcopenia Model.

Methods in molecular biology (Clifton, N.J.)·2024

Related Experiment Video

Updated: Feb 4, 2026

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
06:06

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Published on: July 22, 2021

6.9K

Preparation of Joint Extracts.

Shuang Liu1, Erika Takemasa2

  • 1Department of Pharmacology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. liussmzk@m.ehime-u.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|September 24, 2018
PubMed
Summary

This study details a method for extracting synovium tissue from mice to analyze proteins involved in rheumatoid arthritis pathogenesis. This technique is crucial for understanding arthritis development in mouse models.

Keywords:
Cell isolationJoint tissueMouseProtein extractsSynovium

More Related Videos

Preparation and Fractionation of Xenopus laevis Egg Extracts
07:45

Preparation and Fractionation of Xenopus laevis Egg Extracts

Published on: August 27, 2008

12.6K
Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans
07:22

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

Published on: May 23, 2020

9.9K

Related Experiment Videos

Last Updated: Feb 4, 2026

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
06:06

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Published on: July 22, 2021

6.9K
Preparation and Fractionation of Xenopus laevis Egg Extracts
07:45

Preparation and Fractionation of Xenopus laevis Egg Extracts

Published on: August 27, 2008

12.6K
Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans
07:22

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

Published on: May 23, 2020

9.9K

Area of Science:

  • Rheumatoid Arthritis Research
  • Biomolecular Analysis
  • Animal Models

Background:

  • Mice are essential models for studying rheumatoid arthritis (RA) pathogenesis.
  • Protein analysis is fundamental for understanding RA development at a cellular level.
  • Joint tissue extraction, specifically synovium, is a critical first step in protein analysis.

Purpose of the Study:

  • To introduce a reliable method for harvesting synovium from model mice.
  • To describe the preparation of protein extracts from harvested synovium.
  • To facilitate the assessment of local arthritis pathogenesis in mouse models.

Main Methods:

  • Development of a quadriceps approach for synovium harvesting in mice.
  • Detailed protocol for preparing protein extracts from small, fragile joint tissues.
  • Focus on overcoming challenges associated with mouse synovium retrieval.

Main Results:

  • Successful demonstration of a method for obtaining mouse synovium.
  • Establishment of a procedure for subsequent protein extraction.
  • The described method addresses the technical difficulties of working with small, fragile mouse tissues.

Conclusions:

  • The presented synovium harvesting and protein extraction method is vital for advancing rheumatoid arthritis research in mouse models.
  • This technique enables more accurate assessment of arthritis pathogenesis.
  • Improved methodologies for tissue handling are crucial for molecular analysis in small animal studies.