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Role of Skin in Vitamin D Synthesis01:23

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The skin plays a crucial role in the synthesis of vitamin D, a vital nutrient for various physiological processes in the body. Vitamin D is unique because it can be synthesized in the skin through a series of chemical reactions triggered by exposure to ultraviolet B (UVB) radiation from sunlight.
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin...
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Related Experiment Video

Updated: Feb 28, 2026

Generation of Self-assembled Vascularized Human Skin Equivalents
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Transcriptome-Based Evaluation of Hydrogen Gas Effects for Preventing UVA-Induced Photoaging Using an Artificial Skin

Takeshi Kiyoi1, Shuang Liu2, Kentaro Uchida3

  • 1Department of Pharmacology, School of Medicine, Kanazawa Medical University, Uchinada, Ishikawa, Japan.

Geriatrics & Gerontology International
|February 26, 2026
PubMed
Summary

Hydrogen gas may prevent skin aging caused by UVA radiation by reducing oxidative stress and cellular senescence. This study used artificial skin to show hydrogen’s potential protective effects against UVA damage.

Keywords:
UVA‐induced photoagingartificial skinmolecular hydrogentranscriptomic analysis

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Area of Science:

  • Dermatology and photobiology research.
  • In vitro skin models and transcriptomics.

Background:

  • Ultraviolet A (UVA) radiation causes skin photoaging through oxidative stress.
  • Hydrogen gas (H2) shows potential for preventing photoaging, but mechanisms are unclear.
  • In vitro models offer alternatives to animal testing for studying photoaging.

Purpose of the Study:

  • To evaluate the preventive effects of hydrogen gas on UVA-induced photoaging.
  • To investigate the molecular mechanisms underlying hydrogen's protective effects.
  • To utilize an artificial skin model for short-term in vitro studies.

Main Methods:

  • Artificial skin was exposed to UVA radiation (7 or 10.5 J/cm²/day) for three cycles.
  • Incubation with or without 1.3% hydrogen gas occurred between irradiation periods.
  • Transcriptomic and histological analyses were performed to assess cellular changes.

Main Results:

  • Low-dose UVA (7 J/cm²/day) induced significant transcriptomic alterations related to photoaging.
  • High-dose UVA (10.5 J/cm²/day) caused more severe histological damage but fewer transcriptomic changes.
  • Hydrogen gas modulated UVA-induced NRF2 and NFκB1 pathways and suppressed p53-mediated senescence.

Conclusions:

  • Photoaging-related transcriptomic changes are detectable in artificial skin even with minimal histological damage.
  • Hydrogen gas may protect against UVA-induced cellular stress and senescence.
  • This study supports further research into molecular hydrogen for preventing UVA-induced photoaging.