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Related Concept Videos

Role of Proteins in the Human Body01:28

Role of Proteins in the Human Body

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Proteins are the building block of life. They are also  the most abundant macromolecules with as many diverse roles in the body. They are part of many structural components that provide unique shapes and structures to animal cells, tissues, and organs. In addition, they also act as biological catalysts and carry out several anabolic and catabolic reactions. Notably, some proteins are chemical messengers and regulate many critical processes, such as metabolism, growth, and development. They...
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Ribosome Profiling02:24

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

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Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
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Taping Over Different Ground Profiles01:12

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Taping over varying ground profiles requires careful adaptation to achieve accurate measurements. On smooth, level ground with minimal vegetation, the tape can rest directly on the ground. Here, the taping team, typically consisting of a head and a rear tapeman, coordinates their positions with clear communication. The rear tapeman holds the tape at the starting point and guides the head tapeman toward a range pole placed beyond the endpoint, using hand or voice signals to ensure alignment.On...
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Protein-protein Interfaces02:04

Protein-protein Interfaces

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Protein and Protein Structure02:15

Protein and Protein Structure

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Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
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Related Experiment Video

Updated: Feb 4, 2026

Electrochemiluminescence Assays for Human Islet Autoantibodies
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Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen.

Hiroyuki Takeda1

  • 1Proteo-Science Center, Ehime University, Matsuyama, Japan. takeda.hiroyuki.mk@ehime-u.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|September 24, 2018
PubMed
Summary
This summary is machine-generated.

Autoantibody profiling using a novel cell-free synthesized protein array can sensitively detect disease-related autoantibodies. This technology aids in understanding autoimmune diseases, cancer, and lifestyle diseases for better diagnosis and therapeutics.

Keywords:
AlphaScreenAutoantibodyAutoantibody profilingAutoantigenProtein arrayWheat cell-free protein synthesis

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Area of Science:

  • Biochemistry
  • Immunology
  • Molecular Diagnostics

Background:

  • Autoantibodies targeting self-antigens are implicated in autoimmune diseases, cancer, and lifestyle diseases.
  • Analyzing autoantibodies is crucial for understanding disease mechanisms, diagnosis, and developing targeted therapies.

Purpose of the Study:

  • To develop a sensitive and efficient autoantibody profiling assay.
  • To enable high-throughput screening of autoantibodies in patient sera.

Main Methods:

  • Utilized cell-free synthesized protein arrays for antigen presentation.
  • Employed high-throughput screening technology for sensitive detection of autoantibody-antigen interactions.

Main Results:

  • The developed assay can sensitively detect interactions between recombinant antigen proteins and autoantibodies.
  • The system efficiently analyzes autoantibody profiles in patient serum samples.

Conclusions:

  • The novel autoantibody profiling assay provides a sensitive and efficient tool for disease research.
  • This technology has potential applications in the diagnosis and therapeutic development for autoimmune diseases, cancer, and lifestyle diseases.