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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Exploring the Potential of Mesenchymal Stem Cell Sheet on The Development of Hepatocellular Carcinoma In Vivo
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Mesenchymal Stem Cell Engineering.

Shuang Liu1

  • 1Department of Pharmacology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. liussmzk@m.ehime-u.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|September 24, 2018
PubMed
Summary
This summary is machine-generated.

Mesenchymal stem cells (MSC) show potential for regenerating joint tissues. Gene modification using CRISPR technology could enhance MSC capabilities for treating conditions like rheumatoid arthritis (RA).

Keywords:
CRISPR/Cas9Genomic manipulationMesenchymal stem cellsPuromycin selectionRegenerative potential

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Molecular Biology

Background:

  • Mesenchymal stem cells (MSC) are multipotent cells capable of tissue regeneration.
  • MSC applications include cartilage repair and stimulating endogenous repair in arthritic joints.
  • Enhancing MSC chondrogenesis via gene therapy is a key area for joint regeneration.

Purpose of the Study:

  • To explore the potential of gene-modified MSC for enhanced chondrogenesis.
  • To investigate the use of CRISPR-Cas9 technology for modifying MSC.
  • To assess the therapeutic prospects of modified MSC in treating rheumatoid arthritis (RA).

Main Methods:

  • Utilizing clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) technology for genomic manipulation.
  • Modifying target genes within MSC to enhance chondrogenic potential.
  • Evaluating the efficacy of modified MSC for joint regeneration in preclinical models.

Main Results:

  • Gene-modified MSC demonstrate enhanced capacity for chondrogenesis.
  • CRISPR-Cas9 mediated modification offers a precise method for MSC enhancement.
  • Modified MSC present a promising therapeutic strategy for diseased joints.

Conclusions:

  • Targeted gene modification of MSC using CRISPR-Cas9 is a viable approach for regenerative medicine.
  • Enhanced MSC hold significant therapeutic promise for treating joint diseases like RA.
  • This strategy could lead to novel treatments for cartilage and osteochondral defects.