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Chemical and Biochemical Approach to Make a Perfect Biocatalytic System on Carbonaceous Matrices
Paulina Bolibok1, Katarzyna Roszek2, Marek Wiśniewski1
1Faculty of Chemistry, Physicochemistry of Carbon Materials Research Group, Nicolaus Copernicus University in Toruń, Toruń, Poland.
Abstract:
Enzymatic processes are widely used in food industry, pharmacy, cosmetic and household chemistry, and medicine. However, the common and efficient application of the biological catalysts is limited by a number of factors that influence enzymes activity. One of the most frequent methods to improve the biocatalysts' properties is immobilization. This chapter presents a recent overview of our attempts to obtain the perfect biocatalytic system. The experimental approach, proposed in this chapter, includes the critical points like: the choice of adequate immobilization method, most suitable carrier, determination of enzyme kinetic parameters, stability, and toxicity of obtained systems. As carbon materials including graphene-derived materials offer unique properties and a plenty of different modifications, these parameters seem to be of decisive importance to understand chemistry of complex systems. Consideration of all the mentioned requirements lead us to the conclusion that graphene oxide could be the best candidate for support in perfect biocatalytic systems.
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