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Updated: Feb 4, 2026

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering
Published on: April 25, 2013
Mechanisms and effectivity of sulfate reducing bioreactors using a chitinous substrate in treating mining influenced
Souhail R Al-Abed1, Patricio X Pinto2, John McKernan1
1National Risk Management Research Laboratory, U.S. Environmental Protection Agency, 26 West Martin Luther King Dr, Cincinnati, OH 45268, United States.
Abstract:
Mining-influenced water (MIW) is one of the main environmental challenges associated with the mining industry. Passive MIW remediation can be achieved through microbial activity in sulfate-reducing bioreactors (SRBRs), but their actual removal rates depend on different factors, one of which is the substrate composition. Chitinous materials have demonstrated high metal removal rates, particularly for the two recalcitrant MIW contaminants Zn and Mn, but their removal mechanisms need further study. We studied Cd, Fe, Zn, and Mn removal in bioactive and abiotic SRBRs to elucidate the metal removal mechanisms and the differences in metal and sulfate removal rates using a chitinous material as substrate. We found that sulfate-reducing bacteria are effective in increasing metal and sulfate removal rates and the duration of operation in SRBRs, and that the main mechanism involved was metal precipitation as sulfides. The solid residues provided evidence of the presence of sulfides in the bioactive column, more specifically ZnS, according to XPS analysis. The feasibility of passive treatments with a chitinous substrate could be an important option for MIW remediation.
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