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Control of Aliivibrio fischeri Luminescence and Decrease in Bioluminescence by Fungicides
Hitomi Kuwahara1, Junko Ninomiya1,2, Hiroshi Morita3
1Graduate School of Environment Engineering, University of Kitakyushu.
Biocontrol Science
|September 26, 2018
Summary
Marine luminous bacteria like Aliivibrio fischeri require specific ions (K+, HCO3-, SO42-) for luminescence. These bacteria can serve as biosensors for detecting fungicides such as sodium orthophenylphenol.
Area of Science:
- Microbiology
- Environmental Science
- Biotechnology
Background:
- Bacterial bioluminescence is influenced by factors like cell density and UV irradiation.
- The role of seawater components in marine luminous bacteria's luminescence is not fully understood.
- The application of marine luminous bacteria as biosensors for fungicides requires further investigation.
Purpose of the Study:
- To investigate the relationship between seawater components and the luminescence of Aliivibrio fischeri.
- To analyze the toxicity of fungicides on A. fischeri luminescence.
- To explore the potential of A. fischeri as a biosensor for fungicide detection.
Main Methods:
- Cultivating Aliivibrio fischeri in artificial seawater media with varying ionic compositions.
- Measuring bacterial luminescence under different conditions.
- Assessing the impact of sodium orthophenylphenol on A. fischeri luminescence.
Main Results:
- Luminescence activation in A. fischeri was observed in the presence of KCl, NaHCO3, and MgSO4.
- A. fischeri requires K+, HCO3-, and SO42- ions for cell density-independent luminescence.
- A decrease in luminescence was detected in the presence of sodium orthophenylphenol at concentrations above 10 ppm.
Conclusions:
- Aliivibrio fischeri requires specific ions (K+, HCO3-, SO42-) for luminescence.
- A. fischeri demonstrates sensitivity to the fungicide sodium orthophenylphenol.
- A. fischeri can be utilized as a biosensor for detecting sodium orthophenylphenol.
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