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Assays for phenotypic and functional characterization of cryopreserved platelets
Denese C Marks1,2, Lacey Johnson1
1a Research and Development , Australian Red Cross Blood Service , Sydney , NSW , Australia.
Platelets
|September 26, 2018
Summary
Cryopreservation extends platelet shelf-life but alters platelet phenotype. New testing methods like flow cytometry are needed to assess cryopreserved platelet quality and function accurately.
Area of Science:
- Transfusion Medicine
- Hematology
- Biopreservation
Background:
- Platelet concentrates have a short shelf-life, limiting their availability.
- Cryopreservation can extend platelet shelf-life to over two years.
- Freezing and thawing can damage platelets, altering their characteristics.
Purpose of the Study:
- To evaluate the quality and function of cryopreserved platelets.
- To identify appropriate methodologies for assessing cryopreserved platelet viability.
- To address the challenges posed by altered cryopreserved platelet phenotypes.
Main Methods:
- Phenotypic characterization using flow cytometry.
- Global coagulation assays.
- Comparison with traditional light transmission aggregometry (LTA).
Main Results:
- Cryopreserved platelets exhibit a significantly different phenotype compared to liquid-stored platelets.
- Light transmission aggregometry (LTA) is unsuitable for assessing cryopreserved platelet function due to poor agonist response.
- Flow cytometry and global coagulation assays provide a clearer assessment of cryopreserved platelet phenotype and function.
Conclusions:
- Standard methods like LTA are inadequate for evaluating cryopreserved platelets.
- Flow cytometry and global coagulation assays are recommended for assessing cryopreserved platelet quality.
- Appropriate testing is crucial for the increasing international use of platelet cryopreservation.
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