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Comparative genomics of five different resistance plasmids coexisting in a clinical multi-drug resistant Citrobacter

Jing Ouyang1,2, Fengjun Sun1, Dongsheng Zhou3

  • 1Department of Pharmacy, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China, peiyuan_xia2013@163.com.

Infection and Drug Resistance
|September 27, 2018
PubMed
Abstract

Insights

This study sequenced five resistance plasmids from a multi-drug resistant Citrobacter freundii, revealing 24 resistance genes. This provides insight into how bacteria maintain multiple plasmids and numerous resistance genes.

Area of Science:

  • Microbiology
  • Genomics
  • Antimicrobial Resistance

Background:

  • Plasmid-mediated multi-drug resistance (MDR) is prevalent in Citrobacter freundii.
  • C. freundii P10159 was isolated from a patient with a postoperative urinary tract infection.

Purpose of the Study:

  • To determine the complete nucleotide sequences of five resistance plasmids from C. freundii P10159.
  • To characterize the resistance phenotypes mediated by these plasmids.

Main Methods:

  • High-throughput genome sequencing of five plasmids (pP10159-1 to pP10159-5).
  • Comparative analysis of plasmid sequences.
  • Plasmid transfer experiments.
  • CarbaNP testing for carbapenemase activity.
  • Antimicrobial susceptibility testing.

Main Results:

  • Sequencing revealed five distinct plasmids carrying various resistance genes, including blaNDM-1, blaIMP-4, qnrS1, and blaKPC-2.
  • Three plasmids (pP10159-3, pHS062105-3, pECN49-KPC) carrying blaKPC-2 formed a novel plasmid group.
  • The five plasmids contained a total of 24 genes conferring resistance to 13 different antibiotics or toxic compounds.
  • Plasmids pP10159-4 and pP10159-5 showed backbone similarities to known plasmids but had distinct accessory resistance regions.

Conclusions:

  • This is the first report detailing the complete sequences of five co-occurring resistance plasmids in a single bacterial isolate.
  • The findings offer a deeper understanding of the co-selection and maintenance mechanisms of multiple plasmids and a large number of resistance genes within a single bacterial strain.
Keywords:
Citrobacter freundiimobile elementsmulti-drug resistanceplasmids

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