Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Molecules and Compounds02:38

Molecules and Compounds

68.8K
Atoms and Molecules
68.8K
Self-Evaluation: Self-Enhancement and Self-Verification03:00

Self-Evaluation: Self-Enhancement and Self-Verification

5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
Bioavailability Enhancement: Drug Solubility Enhancement01:16

Bioavailability Enhancement: Drug Solubility Enhancement

261
Body:Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
261
Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

205
Body:After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt...
205
Bioavailability Enhancement: Drug Stability Enhancement and GI Retention01:05

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

219
Body:Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...
219
Local Attraction01:22

Local Attraction

385
Local attraction refers to disturbances in compass readings caused by magnetic influences from nearby objects such as metal fences, buried pipes, vehicles, buildings, power lines, or natural iron ore deposits. Small items like wristwatches, steel tools, or belt buckles can also interfere with the compass by creating local magnetic fields that distort the Earth's natural magnetic field. These distortions lead to inaccurate readings, posing navigation and land surveying challenges.Local...
385

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of β-Lapachone as a Potent USP22 Inhibitor That Suppresses Cancer Stemness and Enhances Chemosensitivity in Lung Adenocarcinoma.

International journal of molecular sciences·2026
Same author

Sensing and Communicating β-Cell Stress in the Context of T1D Etiology: New Opportunities for Therapeutic Impact.

Comprehensive Physiology·2026
Same author

Generation of mixed-valency, modular multispecific antibodies using disulfide-linked Fc-FcγR complexes.

Nature communications·2026
Same author

Colloidal Structure Engineering of Perovskite Precursor for Efficient Pure Blue LEDs with High Chlorine Content.

ACS applied materials & interfaces·2026
Same author

Image Analysis Platform for Comprehensive Quantification of Extracellular Vesicle Morphology.

Proteomics·2026
Same author

Enhancing Fluorescence Lifetime Imaging With Differential Transformer.

Journal of biophotonics·2026
Same journal

A Ni-Mediated Cross-Coupling Approach to Deuterated <sup>18</sup>F- Fluoromethylated (Hetero)arenes.

Journal of the American Chemical Society·2026
Same journal

Efficient Light-Driven CO<sub>2</sub> Capture and Reversible Release Enabled by Metastable Photoacid-Decorated Metal-Organic Frameworks.

Journal of the American Chemical Society·2026
Same journal

In Situ Raman Spectroscopy Reveals the Dynamic Evolution and Ethanol Dependence of SEI Structure in Li-Mediated N<sub>2</sub> Reduction Reaction.

Journal of the American Chemical Society·2026
Same journal

Solvent Esterification and Stoichiometric Control in Ambient-Grown FAPbI<sub>3</sub> Single-Crystal Solar Cells.

Journal of the American Chemical Society·2026
Same journal

Unlocking Azulene Functionalization via Strain-Induced Azulyne Intermediates.

Journal of the American Chemical Society·2026
Same journal

An Oxazine-Locked Covalent Organic Framework by a Tandem Pinner/Schiff Base Reaction for Hydrogen Peroxide Photosynthesis.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Feb 4, 2026

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy
10:57

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Published on: November 11, 2025

816

A Platform To Enhance Quantitative Single Molecule Localization Microscopy.

Ottavia Golfetto1, Devin L Wakefield1, Eliedonna E Cacao1

  • 1Department of Molecular Medicine , Beckman Research Institute, City of Hope , 1500 East Duarte Road , Duarte , California 91010 , United States.

Journal of the American Chemical Society
|September 27, 2018
PubMed
Summary
This summary is machine-generated.

A new Surface Assay for Molecular Isolation (SAMI) improves quantitative single-molecule localization microscopy (qSMLM) for accurate protein quantification. This method enhances the study of endogenous protein organization and its changes in response to therapies.

More Related Videos

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.8K
Analyzing the &#945;-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy
07:02

Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy

Published on: November 3, 2020

7.1K

Related Experiment Videos

Last Updated: Feb 4, 2026

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy
10:57

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Published on: November 11, 2025

816
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.8K
Analyzing the &#945;-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy
07:02

Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy

Published on: November 3, 2020

7.1K

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Quantitative single-molecule localization microscopy (qSMLM) is crucial for in situ protein organization studies.
  • Accurate interpretation of qSMLM data is hindered by uncertainties in fluorescent reporter photophysical properties.
  • Detecting endogenous proteins is challenging due to label heterogeneity and reporter size limitations.

Purpose of the Study:

  • To develop and validate a novel Surface Assay for Molecular Isolation (SAMI) for qSMLM.
  • To characterize photophysical properties of fluorescent reporters using SAMI-qSMLM.
  • To determine the density and nano-organization of endogenous membrane proteins, including EGFR, HER2, and HER3.

Main Methods:

  • Development of the Surface Assay for Molecular Isolation (SAMI) for qSMLM.
  • Characterization of fluorescent protein and dye photophysical properties.
  • Utilized fluorescent ligands with engineered antibody fragments for endogenous protein detection.
  • Employed SAMI, antibody engineering, and pair-correlation analysis for receptor quantification.
  • Investigated receptor nano-organization in breast cancer cell lines.

Main Results:

  • SAMI-qSMLM provided robust quantification of molecular properties.
  • Successfully determined the density and nano-organization of membrane-bound EGFR, HER2, and HER3.
  • Observed distinct changes in receptor density and nano-organization following therapeutic agent treatment in breast cancer cells.

Conclusions:

  • The developed SAMI platform significantly improves molecular quantification in qSMLM.
  • This approach enables efficient detection of endogenous proteins with high specificity.
  • The platform holds potential for studying the local protein environment in intact cells and understanding therapeutic effects.