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lncRNA - Long Non-coding RNAs02:39

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Use of Alu Element Containing Minigenes to Analyze Circular RNAs
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Functional Analysis of Circular RNAs.

Shanmugapriya1, Hisham Alkatib Huda1, Soundararajan Vijayarathna1

  • 1Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang, Malaysia.

Advances in Experimental Medicine and Biology
|September 28, 2018
PubMed
Summary

Circular RNAs (circRNAs) are abundant in the human transcriptome and regulate gene expression. This chapter details methods for functional validation of these important non-coding RNAs using cellular models.

Keywords:
Cellular modelCircRNAsFunctional validation

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Circular RNAs (circRNAs) are a widespread class of endogenous non-coding RNAs formed by back-splicing.
  • While abundant in the human transcriptome, their biological roles are largely unknown.
  • Thousands of circRNAs have been identified across various organisms, suggesting significant biological relevance.

Purpose of the Study:

  • To provide a comprehensive overview of functional validation methods for circRNAs.
  • To discuss recent methodologies for assessing circRNA function.
  • To demonstrate these methods on a cellular model.

Main Methods:

  • RNA interference (RNAi) gene silencing assays.
  • Luciferase reporter assays.
  • circRNA overexpression and RT-qPCR quantification.

Main Results:

  • The chapter details various assays for functional validation of circRNAs.
  • These methods enable the study of circRNA roles in gene expression regulation.
  • Demonstration on a cellular model provides practical insights.

Conclusions:

  • Functional validation is crucial for understanding circRNA roles in biological processes.
  • Modern methodologies offer robust approaches for studying circRNA function.
  • This chapter serves as a guide to circRNA functional validation techniques.