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[[Protective action of biphenyl dimethyl dicarboxylate (DDB) against liver nuclear DNA damage induced by

W Qing, G Liu

    Zhonghua Yi Xue Za Zhi = Chinese Medical Journal; Free China Ed
    |September 28, 2018
    PubMed

    Insights

    DDB demonstrates protective effects against carcinogen-induced DNA damage by inhibiting benzo(a)pyrene binding and aflatoxin B1-induced DNA synthesis. It also enhances protective enzymes in mice.

    Area of Science:

    • Biochemistry
    • Toxicology
    • Molecular Biology

    Context:

    • Carcinogen exposure poses significant health risks due to DNA damage.
    • Understanding protective mechanisms against carcinogens is crucial for disease prevention.

    Purpose:

    • To investigate the protective effects of DDB against carcinogen-induced DNA damage.
    • To evaluate DDB's impact on DNA binding and repair mechanisms.
    • To assess DDB's influence on carcinogen-metabolizing enzymes.

    Summary:

    • DDB (1 mmol/L) inhibited the binding of 3H-benzo(a)pyrene to rat liver nuclear DNA by approximately 60%.
    • DDB dose-dependently inhibited unscheduled DNA synthesis (UDS) in rat hepatocytes induced by aflatoxin B1.
    • Oral DDB administration in mice increased liver cytosol glutathione-S-transferase and microsomal UDP-glucuronosyltransferase activity.

    Impact:

    • DDB exhibits direct and indirect mechanisms to counteract carcinogen-induced DNA damage.
    • Findings suggest DDB's potential as a chemopreventive agent.
    • Further research into DDB's therapeutic applications is warranted.
    Keywords:
    Aflatoxin B1/*TOXICITYAnimalCell NucleusDNA Damage/DRUG EFFECTSDNA Repair/*DRUG EFFECTSDioxoles/*PHARMACOLOGYEnglish AbstractGlucuronosyltransferase/METABOLISMGlutathione Transferase/METABOLISMLiver/*CYTOLOGYLiver/*ENZYMOLOGYMaleMiceRats

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