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Updated: Feb 4, 2026

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
Published on: January 27, 2016
POTENTIATION EFFECTS OF 3-AMINOBENZAMIDE ON DIVALENT CATION-DEPENDENT DNA FRAGMENTATION IN MAMMALIAN CELLS EXPOSED TO
1DEPARTMENT OF MOLECULAR BIOLOGY, WONKWANG UNIVERSITY, IRI, REPUBLIC OF KOREA.
Abstract:
THE PROPERTIES OF METHYL METHANESULFONATE (MMS) DAMAGE-RELATED NUCLEASE ACTIVITY IN CHO AND HELA CELLS WERE STUDIED BY NEUTRAL AND ALKALINE ELUTION TECHNIQUES. THE FRAGMENTATION OF MMS-TREATED DNA WAS DEPENDENT ON THE PRESENCE OF CA++ AND MG++ IONS, AND WAS NOT OBSERVED UPON TREATMENT WITH DIVALENT ION CHELATORS SUCH AS EDTA OR EGTA. THE CA++ AND MG++ -DEPENDENT CONVERSION OF MMS-TREATED SUPERCOILED PLASMID PREVIOUSLY REACTED WITH CHO CELL NUCLEAR EXTRACT TO OPEN CIRCULAR FORM WAS MARKEDLY INCREASED IN A 3-AMINOBENZAMIDE (3-AB) DOSE-DEPENDENT MANNER AS DETERMINED BY AGAROSE GEL ELECTROPHORESIS AND DENSITOMETRIC ANALYSIS. THESE RESULTS SUGGEST THAT THE INHIBITION OF POLY (ADP-RIBOSE) POLYMERASE BY 3-AB RESULTS IN THE ACTIVATION OF THE CA++, MG++ -DEPENDENT ENDONUCLEASE WHICH NONSPECIFICALLY CUTS MMS-TREATED DNA.
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